Ligand Y for mouse Y for rat Y for human concentration receptor receptor receptor (nM) 0.20 0.048 0.29 0.17 0.50 0.11 0.50 0.33 1.0 0.20 0.67 0.50 4.0 0.50 0.89 0.80 10 0.71 0.95 0.91 20 0.83 0.97 0.95 50 0.93 0.99 0.98 Determine the Ka for the human receptor in this binding experiment. human receptor Kd = nM
Ligand Y for mouse Y for rat Y for human concentration receptor receptor receptor (nM) 0.20 0.048 0.29 0.17 0.50 0.11 0.50 0.33 1.0 0.20 0.67 0.50 4.0 0.50 0.89 0.80 10 0.71 0.95 0.91 20 0.83 0.97 0.95 50 0.93 0.99 0.98 Determine the Ka for the human receptor in this binding experiment. human receptor Kd = nM
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![You are a technician in a biochemistry lab running receptor binding experiments. The target membrane-bound receptor has been
partially purified from mouse, rat, and human cell lines. Using the same radioactive ligand in a saturation binding assay for each
species' receptor, you generate the binding data in the table. The dependent variable, Y, is the fraction of binding sites occupied
by the ligand.
Ligand
Y for mouse
Y for rat
Y for human
concentration
receptor
receptor
receptor
(nM)
0.20
0.048
0.29
0.17
0.50
0.11
0.50
0.33
1.0
0.20
0.67
0.50
4.0
0.50
0.89
0.80
10
0.71
0.95
0.91
20
0.83
0.97
0.95
50
0.93
0.99
0.98
Determine the Ka for the human receptor in this binding experiment.
human receptor Kd
nM](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F279d9c1a-8686-4970-a64d-6e4fa13a7ad9%2F31edb347-d657-4aba-9114-e6583cb67da9%2Fvgnlgdz_processed.png&w=3840&q=75)
Transcribed Image Text:You are a technician in a biochemistry lab running receptor binding experiments. The target membrane-bound receptor has been
partially purified from mouse, rat, and human cell lines. Using the same radioactive ligand in a saturation binding assay for each
species' receptor, you generate the binding data in the table. The dependent variable, Y, is the fraction of binding sites occupied
by the ligand.
Ligand
Y for mouse
Y for rat
Y for human
concentration
receptor
receptor
receptor
(nM)
0.20
0.048
0.29
0.17
0.50
0.11
0.50
0.33
1.0
0.20
0.67
0.50
4.0
0.50
0.89
0.80
10
0.71
0.95
0.91
20
0.83
0.97
0.95
50
0.93
0.99
0.98
Determine the Ka for the human receptor in this binding experiment.
human receptor Kd
nM
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