Recall that when we calculated the fraction of estrogen receptors occupied in the presence of 1 nM estradiol and 1 µM BPA, wefound that the fraction of receptors occupied with estradiol was 0.14 and the fraction of receptors occupied with BPA was 0.71.Calculate the concentration of free receptor in terms ofthe total concentration of receptor, Rtotal0.15R totalCalculate the concentration of free BPA in terms of thetotal concentration of receptor, R total.Confirm the approximate value for the dissociationconstant for the estrogen receptor-BPA complex.0.2 nM200 nM5 μ.1 μΜCalculate the concentration of free estradiol in terms ofthe total concentration of receptor, Rtotal1 nM - 0.14R,totalConfirm the approximate value for the dissociationconstant for the estrogen receptor-estradiol complex.

To calculate the concentration of free receptor (Rfree) in terms of the total concentration of…

Answered: Recall that when we calculated the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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MRNA 5' AUG 3' ribosome protein signal kinase transmembrane ligand binding DNA sequence binding domain domain domain domain cytoplasm plasma membrane extracellular fluid
1.Substituents exhibit strong steric repulsion at the GABAA receptor ？what does it means？？ 2. Substitutions at C-6, C-8, and C-9 lower the activity？where is C-6, C-8, and C-9 ？can anyone help to lable out？
2 of 16 Compound Binds to Elicits biological receptor response A Yes Yes B Yes No C No No Refer to the table above. Compound X is the natural ligand that binds to a receptor and stimulates a signal transduction pathway associated with the receptor. Based on the information given in the table, what are the roles of compounds A, B, and C? O A is an antagonist, B is an agonist, and C is a ligand. A is an agonist, B is an antagonist, and C has no role. A is a ligand, B has no role, and C is an antagonist. A is an agonist, B is an antagonist, and C is an agonist. A has no role, B is an agonist, and C is an antagonist.
Plz do Asap...!
2+ Activation of 5-HT2A Increases cytosolic free Ca²+ Concentration ([Ca²+]) Via a Signaling cascade. At rest, neurons have a cytosolic Ca Concentration of 100nM. The ex- Tra cellular free calcium concentration is typically 1.2m M. Calculate the resting potential (at equilibrium) produced by the neuron as a result of the calcium ion concentration gradient. (Report your answer in mV ).
Arrange following the boxed information in sequence regarding the dissociation and reassociation of G proteins following the activation of a G protein coupled receptor. GTP-bound Ga, dissociates from B and y cuhunits Gas exchanges GTP for GDP Gas reassociates with By subunits and the rorentor reforms Binding of the ligand to a G-protein rounled recentor GTP-bound Gas binds and activates adenvlul rurlase GTP is hydrolyzed and GDP bound Gas suhunit dissoriates Conformational change Increased synthesis of CAMP from ATP
5.. A sample of cells has a total receptor concentration of 25 mM. Ninety percent of the receptors have bound ligand and the concentration of free ligand is 125 µM. Calculate the K for the receptor ligand interaction. (a) What is the AG° for binding for this interaction at 25°C?
Researchers who study those rods cells have performed a series of experiments to better understand this phototransduction pathway and the role of this PDE protein in patients that suffer from night blindness. In one experiment, researchers have measured the cytosolic concentration of cGMP (cyclic- GMP) under different light intensities (exposure = 10 min) in both the presence and the absence of the PDE6i inhibitor. The results are as follow: the cytosolic concentration of cGMP (cyclic-GMP) decreases as light intensity increases, but only in the absence of the PDE6i inhibitor (see figure below). Without PDE6i cGMP concentration (A.U) 25 20 15- 0- 0 0.1 0.2 0.3 Light intensity (A.U) 25 0.4 20 15 - 10 5 0 GMP concentration (A.U) cGMP concentration (A.U) 25 20 15- o 0- 0 0.1 With PDE6i 0.2 0.3 Light intensity (A.U) 0.4 25 20 0 GMP concentration (A.U) 10. What is the likely function of the cytosolic cGMP in this phototransduction pathway? A. It is a competitive inhibitor of PDE B. It is a…
why is there a difference between the stock solution concetration of chymotrypsin calculated from zero time y intercepts versus the stock solution label information?
fill in the blanks In signal transduction, termination of the active Gas is by ( ) and termination of the cAMP signal is by ( ).
distribution of Benzene in a 4-compartment PBK model after an exposure of 6h. Convert Berkeley Madonna code to Matlab. Note: The parameters in the table below are for a mouse model. 1a) What are the two type of products containing Benzene consumers were recently concerned about. Which of these two different type of products is likely more harmful? (hint: check news/reports and social media channels) 1b) Plot Jresp along with the concentrations of Benzene in blood, fat, non-fat and liver in a 24h window (pdf). Why is Jresp slightly decreasing within 6h of exposure? Here is the Berkeley Madonna code: {Top model} METHOD RK4 STARTTIME = 0 STOPTIME = 24 DT = 0.02 {Reservoirs Blood, Fat, NonFat and Liver} d/dt (NF) = + Jnf INIT NF = 0 d/dt (F) = + Jf INIT F = 0 d/dt (B) = - Jnf - Jf - Jl + Jresp INIT B = 0 d/dt (L) = + Jl - Jmetab INIT L = 0 {Flows} Jnf = Qnf*(Cb-Cnf) Jf = Qf*(Cb-Cfv) Jl = Ql*(Cb - Clv) Jmetab = vmax*Cl/(Km +…
The dissociation equilibrium constant (Kd) is:_____ (select all that apply) Group of answer choices: Is a measure of ligand efficacy Typically equivalent to 1- Ka The concentration of ligand that gives maximal receptor occupancy The concentration of ligand that gives half-maximal receptor occupancy Measured in 1/M or M-1 Typically measured in mol/L or M A measure of the strength of a ligand - receptor binding reaction

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