Use Excel to make the data plot and include the graph in your submission. For all questions requiring calculations, show your work, include 2 significant digits after the decimal and include units. Place a box around your final answer. Include a title and axes on all plots. 1. Mammals use two types of G-protein coupled receptors (GPCRs) to detect sweet, umami and bitter tastes. Umami peptides bind the bitter taste receptor at an allosteric site and suppress receptor activation, even in the presence of a bitter molecule. For questions a-e below consider the interaction between the bitter taste receptor protein, hTAS2R16, and the umami peptide, Glu-Glu. a. Plot the [Glu-Glu] vs θ in excel. Add an image of this graph to your submission. b. Looking at the graph, estimate the Kd for the interaction between hTAS2R16 receptor and the Glu-Glu ligand complex. c. Calculate the Kd for the hTAS2R16 receptor and the Glu-Glu ligand complex. d. Suppose a N96A mutation in hTAS2R16 causes a 6.50-fold decrease in affinity. Plot a binding curve for the mutant binding curve on the same graph in part a (submit this as a second graph). e. Hypothesize why a N96A mutation in the receptor confers decreased affinity for the ligand. (Consider the properties of the N and A sidechains and what type of non-covalent interactions they might make with the Glu-Glu dipeptide). [Glu-Glu] (mM) Θ 0.53 0.0579 1.12 0.1150 2.71 0.2392 6.42 0.4269 11.16 0.5642 20.58 0.7048 35.78 0.8059 64.08 0.8814 90.56 0.9131 120.77 0.9334
Use Excel to make the data plot and
include the graph in your submission. For all questions requiring calculations, show your work,
include 2 significant digits after the decimal and include units. Place a box around your final answer.
Include a title and axes on all plots.
1. Mammals use two types of G-protein coupled receptors
(GPCRs) to detect sweet, umami and bitter tastes. Umami peptides
bind the bitter taste receptor at an allosteric site and suppress
receptor activation, even in the presence of a bitter molecule. For
questions a-e below consider the interaction between the bitter taste
receptor protein, hTAS2R16, and the umami peptide, Glu-Glu.
a. Plot the [Glu-Glu] vs θ in excel. Add an image of this graph to
your submission.
b. Looking at the graph, estimate the Kd for the interaction between
hTAS2R16 receptor and the Glu-Glu ligand complex.
c. Calculate the Kd for the hTAS2R16 receptor and the Glu-Glu ligand complex.
d. Suppose a N96A mutation in hTAS2R16 causes a 6.50-fold decrease in affinity. Plot a binding
curve for the mutant binding curve on the same graph in part a (submit this as a second
graph).
e. Hypothesize why a N96A mutation in the receptor confers decreased affinity for the ligand.
(Consider the properties of the N and A sidechains and what type of non-covalent interactions
they might make with the Glu-Glu dipeptide).
[Glu-Glu] (mM) Θ
0.53 0.0579
1.12 0.1150
2.71 0.2392
6.42 0.4269
11.16 0.5642
20.58 0.7048
35.78 0.8059
64.08 0.8814
90.56 0.9131
120.77 0.9334
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