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The following images represent confocal images of cells with full-length Mira protein (Mira 1-830), which localizes to the plasma membrane, and truncations of the Mira protein that either localize to the plasma membrane and/or the nucleus. How would you determine whether the membrane localization sequence of Mira is sufficient? ira 1-830 Mra 1-727 Ma 14CS Mira 1-106 Mia 14 Mi 1329 Mirs 1-290 M 420 Remove amino acids 94-290 from Mira and examine the localization of the protein Remove amino acids 1-106 from Mira and examine the localization of the protein Add amino acids 1-830 from Mira to GFP and examine localization of GFP Add amino acids 94-290 from Mira to GFP and examine localization of GFP O Add amino acids 1-106 from Mira to GFP and examine localization of GFP

The following images represent confocal images of cells with full-length Mira protein (Mira 1-830), which localizes to the plasma membrane, and truncations of the Mira protein that either localize to the plasma membrane and/or the nucleus. How would you determine whether the membrane localization sequence of Mira is sufficient? ira 1-830 Mra 1-727 Ma 14CS Mira 1-106 Mia 14 Mi 1329 Mirs 1-290 M 420 Remove amino acids 94-290 from Mira and examine the localization of the protein Remove amino acids 1-106 from Mira and examine the localization of the protein Add amino acids 1-830 from Mira to GFP and examine localization of GFP Add amino acids 94-290 from Mira to GFP and examine localization of GFP O Add amino acids 1-106 from Mira to GFP and examine localization of GFP

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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**Transcription for Educational Website:** **Title: Determining the Membrane Localization Sequence of Mira Protein** The images below are confocal images of cells expressing the full-length Mira protein (Mira 1-830), which localizes to the plasma membrane. The images also include truncations of the Mira protein that localize either to the plasma membrane and/or the nucleus. The primary objective here is to determine the sufficiency of the membrane localization sequence of Mira. **Diagram Description:** A series of confocal images are labeled A through H: - **A (Mira 1-830):** Displays the full-length Mira protein, showing localization at the plasma membrane. - **B (Mira 1-727):** Shows a truncation of the protein, localizing similarly to the full-length protein. - **C (Mira 1-648):** Another truncation indicating similar localization. - **D (Mira 1-465):** Depicts further truncation, with localization primarily at the plasma membrane. - **E (Mira 1-329):** Shows localization changes, potentially to both the plasma membrane and other areas. - **F (Mira 1-280):** Indicates nuclear localization for this truncation. - **G (Mira 1-106):** Primarily nuclear localization is observed. - **H (Mira 94-290):** Exhibits distinct localization, differing from the others. **Options to Determine Membrane Localization Sequence Sufficiency:** 1. Remove amino acids 94-290 from Mira and examine the localization of the protein. 2. Remove amino acids 1-106 from Mira and examine the localization of the protein. 3. Add amino acids 1-830 from Mira to GFP and examine localization of GFP. 4. Add amino acids 94-290 from Mira to GFP and examine localization of GFP. 5. Add amino acids 1-106 from Mira to GFP and examine localization of GFP. Using these experimental approaches, one can assess the sequences necessary for proper membrane localization of the Mira protein.
Transcribed Image Text:**Transcription for Educational Website:** **Title: Determining the Membrane Localization Sequence of Mira Protein** The images below are confocal images of cells expressing the full-length Mira protein (Mira 1-830), which localizes to the plasma membrane. The images also include truncations of the Mira protein that localize either to the plasma membrane and/or the nucleus. The primary objective here is to determine the sufficiency of the membrane localization sequence of Mira. **Diagram Description:** A series of confocal images are labeled A through H: - **A (Mira 1-830):** Displays the full-length Mira protein, showing localization at the plasma membrane. - **B (Mira 1-727):** Shows a truncation of the protein, localizing similarly to the full-length protein. - **C (Mira 1-648):** Another truncation indicating similar localization. - **D (Mira 1-465):** Depicts further truncation, with localization primarily at the plasma membrane. - **E (Mira 1-329):** Shows localization changes, potentially to both the plasma membrane and other areas. - **F (Mira 1-280):** Indicates nuclear localization for this truncation. - **G (Mira 1-106):** Primarily nuclear localization is observed. - **H (Mira 94-290):** Exhibits distinct localization, differing from the others. **Options to Determine Membrane Localization Sequence Sufficiency:** 1. Remove amino acids 94-290 from Mira and examine the localization of the protein. 2. Remove amino acids 1-106 from Mira and examine the localization of the protein. 3. Add amino acids 1-830 from Mira to GFP and examine localization of GFP. 4. Add amino acids 94-290 from Mira to GFP and examine localization of GFP. 5. Add amino acids 1-106 from Mira to GFP and examine localization of GFP. Using these experimental approaches, one can assess the sequences necessary for proper membrane localization of the Mira protein.
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