Previously Protein A and 5mM of ligand X were combined in a neutral solution and 36% of Protein A was ligand bound. Now Protein A and ligand X are combined in an acidic solution. You now find that when there’s a 4 mM concentration of free ligand X, 45% of the protein is bound to ligand. How does a change in pH affect protein A’s affinity for ligand X? a. Protein A has a higher affinity for ligand X in acidic environments b. Protein A has a lower affinity for ligand X in acidic environments c. The solution pH does not affect the affinity of protein A for ligand X. d. The solution pH does not affect the affinity of a protein for a li
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
c. The solution pH does not affect the affinity of protein A for ligand X.
In this scenario, we are comparing how Protein A interacts with ligand X under two different conditions: a neutral solution and an acidic solution. In the neutral solution, 36% of Protein A binds to ligand X when the ligand concentration is 5mM. In the acidic solution, 45% of Protein A binds to ligand X when the ligand concentration is 4mM.
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