In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to a protein.  The intensity of blue color (measured by absorbance of light at a wavelength of 595nm) is proportional to protein concentration. Protein (ug) Absorbance at 595nm 0.00 0.466 9.36 0.676 18.72 0.883 28.08 1.086 37.44 1.280   Use Excel to determine the equation of the line in the format of y = mx + b. (I tried y=0.0218x+0.4706 and it was wrong) Using the calibration curve for the Bradford protein determination in the previous question, determine the number of micrograms of protein in an unknown if it gave an absorbance of 0.973.  I tried 23.505 and it was wrong

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to a protein.  The intensity of blue color (measured by absorbance of light at a wavelength of 595nm) is proportional to protein concentration.

Protein (ug) Absorbance at 595nm
0.00 0.466
9.36 0.676
18.72 0.883
28.08 1.086
37.44 1.280

 

Use Excel to determine the equation of the line in the format of y = mx + b.

(I tried y=0.0218x+0.4706 and it was wrong)

Using the calibration curve for the Bradford protein determination in the previous question, determine the number of micrograms of protein in an unknown if it gave an absorbance of 0.973. 

I tried 23.505 and it was wrong
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