In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to a protein. The intensity of blue color (measured by absorbance of light at a wavelength of 595nm) is proportional to protein concentration. Protein (ug) Absorbance at 595nm 0.00 0.466 9.36 0.676 18.72 0.883 28.08 1.086 37.44 1.280 Use Excel to determine the equation of the line in the format of y = mx + b. (I tried y=0.0218x+0.4706 and it was wrong) Using the calibration curve for the Bradford protein determination in the previous question, determine the number of micrograms of protein in an unknown if it gave an absorbance of 0.973. I tried 23.505 and it was wrong
In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to a protein. The intensity of blue color (measured by absorbance of light at a wavelength of 595nm) is proportional to protein concentration. Protein (ug) Absorbance at 595nm 0.00 0.466 9.36 0.676 18.72 0.883 28.08 1.086 37.44 1.280 Use Excel to determine the equation of the line in the format of y = mx + b. (I tried y=0.0218x+0.4706 and it was wrong) Using the calibration curve for the Bradford protein determination in the previous question, determine the number of micrograms of protein in an unknown if it gave an absorbance of 0.973. I tried 23.505 and it was wrong
Chapter11: Health Of Aquatic Animals
Section: Chapter Questions
Problem 3KA
Related questions
Question
In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to a protein. The intensity of blue color (measured by absorbance of light at a wavelength of 595nm) is proportional to protein concentration.
| Protein (ug) | Absorbance at 595nm |
| 0.00 | 0.466 |
| 9.36 | 0.676 |
| 18.72 | 0.883 |
| 28.08 | 1.086 |
| 37.44 | 1.280 |
Use Excel to determine the equation of the line in the format of y = mx + b.
(I tried y=0.0218x+0.4706 and it was wrong)
Using the calibration curve for the Bradford protein determination in the previous question, determine the number of micrograms of protein in an unknown if it gave an absorbance of 0.973.
I tried 23.505 and it was wrong
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Physiology: From Cells to Systems (MindTap …
Biology
ISBN:
9781285866932
Author:
Lauralee Sherwood
Publisher:
Cengage Learning
Human Physiology: From Cells to Systems (MindTap …
Biology
ISBN:
9781285866932
Author:
Lauralee Sherwood
Publisher:
Cengage Learning
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:
9781305389892
Author:
Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:
Cengage Learning
Human Heredity: Principles and Issues (MindTap Co…
Biology
ISBN:
9781305251052
Author:
Michael Cummings
Publisher:
Cengage Learning