We want to measure the activity of alanine aminotransferase (ALAT) present in aserum. The reaction catalyzed by the enzyme is:Reaction 1:I-*H₂N-glutamateH-C-COOCH₂CH₂COO0.1 M phosphate buffer pH 7.4 : 550 μL1.2 M alanine: 100 μLCH3pyruvateCH3Ctime (min)A340CIOOCOOThe enzyme reaction is alized in the following conditions:In a 1 cm-cuvette are added:COO™pyruvatelactate dehydrogenase* (LDH, 300 µμg.mL-¹): 50 μL1.5 mM NADH : 200 μL0.04 M a-ketoglutarate: 500 µLserum containing ALAT: 600 μµLALATNADH + H+00.915a-cétoglutarateCOOLDH* Lactate dehydrogenase (LDH) reduces pyruvate into lactate, with the concomitantoxydation of NADH. This allows to indirectly measure the amount of product formed.с=0CH₂10.741Reaction 2:NAD+CH₂COO™H-CCH3OHCOO™lactatealanineH+ *H3N-CThe reaction is performed at 25 °C and the absorbance at 340 nm is monitored everyminute, for 5 min. The absorbance values are given in the table below:Data:ENADH at 340 nm = 6220 M-¹1.cm-1.One international unit corresponds to the amount of enzyme that catalyzes theconversion of 1 µmole of substrate to product in one minute.320.572 0.402CH340.233COO50.159=Draw the graph A340 f(t). Comment the shape of the curveCalculate alanine, LDH, NADH and a-ketoglutarate concentrations in the reactionmedium at t = 0 (in the same units as the initial concentrations).Calculate the initial rate Vo of the reaction in M.s¹.

If a particular reaction brings about a measurable change (like the generation or consumption of a…

Answered: - Draw the graph A340 = f(t). Comment…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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- Draw the graph A340 = f(t). Comment the shape of the curve Calculate alanine, LDH, NADH and a-ketoglutarate concentrations in the reaction medium at t = 0 (in the same units as the initial concentrations). Calculate the initial rate V₁ of the reaction in M.s¹.