19. The picture below indicates a Lineweaver-Burk plot used to illustrate the values of KM and Vmax of an enzyme-catalyzed reaction. Please identify what each of the letters (A-E) indicates on the plot (A and D are the labels for each axis and B and E are where the line best fitting kinetic data (indicated in red) intercept the two axes). C (slope) E A B D 20. During the metabolism of ethyl alcohol, electrons are transferred from the alcohol to a NAD molecule (forming NADH and acetaldehyde) by enzyme 1; the acetaldehyde donates another pair of electrons to another NAD+ molecule to form acetic acid or acetate (more correct since it won't be protonated at physiological pH) (catalyzed by enzyme 2). The acetic acid is then added onto a CoA molecule by enzyme 3, forming a thioester bond and the product molecule is known as Acetyl-CoA which enters normal metabolism. What types of reactions (oxidoreductase, hydrolase, transferase, etc.) are carried out by enzymes 1, 2, and 3, respectively?
19. The picture below indicates a Lineweaver-Burk plot used to illustrate the values of KM and Vmax of an enzyme-catalyzed reaction. Please identify what each of the letters (A-E) indicates on the plot (A and D are the labels for each axis and B and E are where the line best fitting kinetic data (indicated in red) intercept the two axes). C (slope) E A B D 20. During the metabolism of ethyl alcohol, electrons are transferred from the alcohol to a NAD molecule (forming NADH and acetaldehyde) by enzyme 1; the acetaldehyde donates another pair of electrons to another NAD+ molecule to form acetic acid or acetate (more correct since it won't be protonated at physiological pH) (catalyzed by enzyme 2). The acetic acid is then added onto a CoA molecule by enzyme 3, forming a thioester bond and the product molecule is known as Acetyl-CoA which enters normal metabolism. What types of reactions (oxidoreductase, hydrolase, transferase, etc.) are carried out by enzymes 1, 2, and 3, respectively?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:19. The picture below indicates a Lineweaver-Burk plot used to illustrate the values of KM and Vmax
of an enzyme-catalyzed reaction. Please identify what each of the letters (A-E) indicates on the
plot (A and D are the labels for each axis and B and E are where the line best fitting kinetic data
(indicated in red) intercept the two axes).
C
(slope)
E
A
B
D
20. During the metabolism of ethyl alcohol, electrons are transferred from the alcohol to a NAD
molecule (forming NADH and acetaldehyde) by enzyme 1; the acetaldehyde donates another
pair of electrons to another NAD+ molecule to form acetic acid or acetate (more correct since it
won't be protonated at physiological pH) (catalyzed by enzyme 2). The acetic acid is then added
onto a CoA molecule by enzyme 3, forming a thioester bond and the product molecule is known
as Acetyl-CoA which enters normal metabolism. What types of reactions (oxidoreductase,
hydrolase, transferase, etc.) are carried out by enzymes 1, 2, and 3, respectively?
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