Glucose oxidase [S] (glucose, mM) 0.1 5 7 8 15 20 0.2 0.4 0.5 1.0 2.0 3.0 5.0 7.5 35 45 55 60 80 90 95 V (umol/min/mg) 10.0 12.0 14.0 Using this data, do an M/M plot. Does this enzyme follow classical M/M kinetics or is there evidence of allosterism?

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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Glucose oxidase
[S] (glucose, mM)
0.1
5
7
8
15
20
0.2
0.4
0.5
1.0
2.0
3.0
5.0
7.5
35
45
55
60
80
90
95
V (μmol/min/mg)
10.0
12.0
14.0
Using this data, do an M/M plot.
Does this enzyme follow classical M/M kinetics or is there
evidence of allosterism?
Transcribed Image Text:Glucose oxidase [S] (glucose, mM) 0.1 5 7 8 15 20 0.2 0.4 0.5 1.0 2.0 3.0 5.0 7.5 35 45 55 60 80 90 95 V (μmol/min/mg) 10.0 12.0 14.0 Using this data, do an M/M plot. Does this enzyme follow classical M/M kinetics or is there evidence of allosterism?
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