Given the kinetic data below, calculate the KM, in mM, of the hypothetical enzyme for substrate R.

Q: An enzyme catalyzes a reaction at a velocity of 20 micromole/min when the concentration of substrate…

A: The Michaelis-Menten equation represents the relationship between substrate concentration and…

Q: The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

Q: 3. Draw the structure of the catalytic triad found in the active site of chymotrypsin and explain…

A: Lineweaver Burk (LB) plot has 1/v0 as Y-axis and 1/[S0] as X-axis. Competitive, Uncompetitive and…

Q: The enzyme BURT was found to function at V0=250 uM/min with a Km=15.0 uM when [S]=10.0 uM. What is…

A: Enzymes are protein molecules that increase the rate of reactions by decreasing the activation…

Q: The turnover number for an enzyme that approximates Michaelis-Menten kinetics is known to be 500…

A: Turn over number of an enzyme is the maximum number of substrate molecules that are converted into…

Q: different substrate (Substrate 2) with a Km = 30 µM (assuming that both substrates give the same…

A: Given that,Vmax for both substrate 1 and substrate 2 is same (95 μM/sec) and Km for substrate 2 is…

Q: The equil ibrium constant for the attachment of a substrate to the active site of an enzyme was…

A: The equilibrium constant of a chemical reaction is the value of its reaction quotient at chemical…

Q: Most of the enzyme reactions followed the mathematical kinetic plots suggested by Michaelis-Menten…

A: The Michaelis- Menten equation is an equation used to predict the rate of product formation of a…

Q: An enzyme-catalyzed reaction is allowed to proceed for 3.4 minutes under steady-state conditions…

A: Ans- Concentration of Product = 6.3uM           Time for which the reaction has been…

Q: Vmax and Km were calculated for enzyme substrate data. Results Y=0,0063x+0,3929 Km =62,4 mikromol/L…

A: From the LB plot of enzyme kinetics, y=mx+c  It's an equation of a straight line. 1/V=(Km/Vm )1/S…

Q: Most of the enzyme reactions followed the mathematical kinetic plots suggested by Michaelis-Menten…

A: Introduction: Those substances that increase the pace of the reactions that occurs within the living…

Q: Sketch the complete reaction free energy diagram for an enzyme-catalyzed conversion of a single…

A: The reactants must overcome a kinetic barrier to proceed with the conversion to products in every…

Q: If a michaelis-type enzyme has an apparent Km of 2*10^-8 and apparent Vmax of 1*10^-4 moles/min in…

A: Uncompetitive inhibitors bind only to the enzyme–substrate complex and reduce both kcat and Km (the…

Q: An enzyme catalyzes a reaction with a Km of 9.50 mM and a Vmax of 2.10 mM s-. Calculate the reaction…

A: Most of the enzymes follow Michalis-Menten enzyme kinetics. In this equation, Vmax is the maximum…

Q: You have two different enzymes, each with 2 substrates, S1 and S2, and 2 products, P1 and P2. A.…

A: There are three major reaction mechanisms seen with enzymes that catalyze a reaction involving 2…

Q: Assume that the reaction catalyzed by an enzyme follows Michaelis-Menten kinetics. The substrate…

A: Mathematical equation of the Michaelis-Menten kinetics is as follows:   V=VmaxSKm+Swhere V =velocity…

Q: Xanthine oxidase (XO) is the enzyme that catalyzes the synthesis of uric acid, which in excess buses…

A: Kaemferol inhibitory effect on Xanthine oxidase: Enzymatic assays have shown that kaempferol…

Q: For the following two scenarios, sketch the complete reaction free energy diagram for an…

A: The enzymes are involved in increasing the reaction rate of a biochemical reaction. A reaction is…

Q: Draw the Michaelis-Menten Plot and Lineweaver-Burke Plot of an enzyme in the following situation.…

A: Enzymes are biocatalysts which increase the rate of biochemical reactions. Enzymes will not…

Q: An enzyme has a Km of 10 mM and a Vm of 100 mmol/min If [S]=10 mM, which will increase the velocity…

A: For a one-substrate enzyme catalyzed reaction, the Michaelis-Menton equation shows the quantitative…

Q: KM is determined by measuring the reaction velocity of two enzymes (X and Y) at different…

A: Enzymes are usually protein molecules which catalyzes a biochemical reaction by decreasing its…

Q: The catalytic efficiency of many enzymes depends on pH.  Chymotrypsin, which has a well-known…

A: Enzymes are high molecular-weight protein molecules that catalyse biochemical reactions. The…

Q: A particular enzyme has a ΔΔG‡ of -22.1 kJ mol-1 at 37.0 °C. Calculate the rate enhancement of this…

A: where:- k is the rate constant,- k_B is the Boltzmann constant,- T is the temperature in Kelvin,- h…

Q: Imagine a typical enzyme kinetic curve of [S] vs. V. The maximum [S] is about four times the Km.…

A: 1. Vmax: A parameter in enzyme kinetics, maximum velocity, or Vmax for short, is the fastest rate at…

Q: please explain the question, i am unable to input values of x

A: The dependent variable, the reaction rate (µmol/L/min), is usually plotted on the y-axis and is…

Q: At what substrate concentration would an enzyme with a kcat of 25.0 s-1 and a KM of 3.5 mM operate…

A: This answer provides a detailed explanation of how to calculate the substrate concentration at which…

Q: A particular enzyme-catalyzed reaction has an apparent Vmax = 9.00 nmol s-1 and α' = 3.00 when 2.00…

A: Approach to solving the question: Detailed explanation:To calculate the Vmax for the uninhibited…

Q: From a kinetics experiment, Kcat was determined to be 55sec^-1. For the kinetic assay, 0.05mL of a…

A: The concentration of enzyme stock solution was 0.05mg/ml. i.e. 1ml contain 0.05mg of enzyme. We took…

Q: Draw a hypothetical Michaelis-Menten plot for an enzyme reaction (i) without and (ii) with a pure…

A: Enzymes are the class of proteins that elevates the rate of the chemical reaction within the living…

Q: Sketch a typical enzyme kinetic curve of [S] vs. V. The maximum [S] should be about four times the…

A: In a typical enzyme kinetic curve, the substrate concentration, [S] is plotted along the x-axis and…

Q: Table 1 shows the kinetic data that have been obtained for glucoamylase from Aspergillus niger at…

A: According to Michaelis-Menten Kinetics, when the rate or velocity of an enzyme catalyzed reaction…

Q: Consider an enzyme that catalyzes the reaction S2 P, by the following simple reaction mechanism: k…

A: The enzyme is the catalyst that increases the rate of reaction by decreasing the activation energy.…

Q: The reaction: A + B P + Q is catalysed by enzyme E. Draw a Cleland diagram for a Non-sequential…

A:

Q: The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial…

A: Those proteins or biological catalysts which help to speed up the chemical reaction are termed…

Q: You were asked to determine the mode of inhibition exerted by Inhibitor DEDS to a newly discovered…

A: Lineweaver-Burk plot, also known as double-reciprocal plot, is the graph plotted for Lineweaver-Burk…

Q: You are working on an enzyme that obeys standard Michaelis-Menten kinetics. You have determined the…

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Q: For an enzyme catalyzed reaction of the form: S + E → P + E, the rate of product formation, [P], is…

A: Km is Michael's constant which is equal to substrate concentration at half of maximum velocity of…

Q: Derive the rate law for the following enzyme reaction chain. Linear the equation you have derived as…

A: Rate law is the rate of chemical…

Q: From a kinetics experiment, the Vmax was determined to be 450µM∙min-1.  For the kinetic assay, 0.1mL…

A: First we need to calculate the total enzyme concentration ( [Et] ) in the system.Stock solution of…

Q: After purifying alkaline phosphatase, you perform enzyme kinetic experiments with and without an…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

Q: The Vmax for a particular enzyme is 10 nmols/L/s. The Km for its substrate is 5 microM. If the…

A: Given that, the Vmax for a particular enzyme is 10 nmol/L/s. The Km for its substrate is 5 uM. We…

Q: Below is kinetic data obtained for an enzyme-catalyzed reaction. The enzyme concentration is fixed…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation energy…

Q: a particular enzyme catalyzes a single reactant S to a single product P, following michaelis-menten…

A: For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…