different substrate (Substrate 2) with a Km = 30 µM (assuming that both substrates give the same Vmax), if [Enzyme] = 0.05 µM, calculate kcat for Substrate 1 and what is the catalytic efficiency in units of M-1s-1?
different substrate (Substrate 2) with a Km = 30 µM (assuming that both substrates give the same Vmax), if [Enzyme] = 0.05 µM, calculate kcat for Substrate 1 and what is the catalytic efficiency in units of M-1s-1?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Substrate 1 is plotted on the following kinetic data, the Vmax and Km for Substrate 1 are 95 uM/sec and 10 µM, respectively. Draw a curve for the data you would expect to observe for a different substrate (Substrate 2) with a Km = 30 µM (assuming that both substrates give the same Vmax), if [Enzyme] = 0.05 µM, calculate kcat for Substrate 1 and what is the catalytic efficiency in units of M-1s-1?
![Velocity (uM/sec)
200
180
160
140
120
100
80
60
40
20
0
0
50
100
[Substrate 1] (μM)
150
200](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F277d8e9e-796c-4923-a9a2-4c05d5ad95df%2F1c2a4a4f-dd90-4910-b57a-b2a1def8d2cd%2Fhgbxvgl_processed.png&w=3840&q=75)
Transcribed Image Text:Velocity (uM/sec)
200
180
160
140
120
100
80
60
40
20
0
0
50
100
[Substrate 1] (μM)
150
200
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 3 steps with 1 images
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON