In the same kinetics experiment above without the inhibitor, what is the fraction of occupied enzyme at substrate concentration 1microM ? How about when it's 1mM ?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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In the same kinetics experiment above without the inhibitor, what is the fraction of occupied enzyme at substrate concentration 1microM ? How about when it's 1mM ?

[S]
Vo w/o inhib
(mM)
2.5 x 10-3
(μmol/min)
28
Vo w/ inhib
(μmol/min)
13
4 x 10-3
40
0.01
70
20
22
38
0.02
95
50
0.04
112
70
0.1
128
90
2
139
115
3579
139.5
125
139.5
132
140
137
10
140
139
Transcribed Image Text:[S] Vo w/o inhib (mM) 2.5 x 10-3 (μmol/min) 28 Vo w/ inhib (μmol/min) 13 4 x 10-3 40 0.01 70 20 22 38 0.02 95 50 0.04 112 70 0.1 128 90 2 139 115 3579 139.5 125 139.5 132 140 137 10 140 139
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