The following data were collected for an enzyme that can be described by MichaelisMenton kinetics. Use graphical analysis to determine the Km and Vmax for this exzyme /substrate combination.[S] mMProduct formed(umol/min)0.211.52.00.243.00.284.00.338.00.4016.00.45

In-order to get accurate Km and Vmax values, we need to draw the LB plot. The LB plot has 1V (i.e.…

Answered: The following data were collected for…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Below is kinetic data obtained for an enzyme-catalyzed reaction. The enzyme concentration is fixed at 100 nM. Using a Lineweaver-Burke plot, calculate the kcat value for this reaction. Report your answer to three significant figures in units of 1/sec.

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The following data were collected for an enzyme that can be described by Michaelis Menton kinetics. Use graphical analysis to determine the Km and Vmax for this exzyme / substrate combination. [S] mM Product formed (umol/min) 0.21 1.5 2.0 0.24 3.0 0.28 4.0 0.33 8.0 0.40 16.0 0.45