For an enzyme-catalyzed reaction, the velocity was determined at two different concentrations of the substrate. Estimate the value of \( V_{\text{max}} \).| [S] (mM) | V (nmol/min) ||----------|--------------|| 10 | 27 || 20 | 48 |Options:- ○ 88 nmol/min- ○ 79 nmol/min- ○ Not enough information is given to form a reasonable estimation.- ○ 67 nmol/min- ○ 51 nmol/min

The velocity of an enzyme catalyzed reaction is given by: V=Vmax×SKM+SWhere,V=velocity of the…

Answered: For an enzyme-catalyzed reaction, the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The rates at various substrate concentrations for an enzyme-catalysed reaction (b) are recorded as follows: 2.5 5.0 10.0 15.0 (S]/10 M 2.2 3.8 5.9 7.1 Vo/ M min Assuming the reaction obeys Michaelis-Menten kinetics, calculate the following values by using Lineweaver-Burk plot () maximum rate, Vmax- (i) Michaelis constant, Km- (ii) turnover number, kz.
From a kinetics experiment, Kcat was determined to be 55sec^-1. For the kinetic assay, 0.05mL of a 0.05mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 30,000g/mole. Assume a reaction volume of 3mL. Calculate Vmax (um*min^-1) for the enzyme and catalytic efficiency (in M^-1sec^-1) for the enzyme. The Km for the enzyme was determined to be 8.3*10^-2M.
5) In an experiment to investigate the inhibition of the enzyme-glucosidase the following data for the rates of reaction with glucopyranoside for various substrate concentrations was obtained. By constructing a Leaver-Burk plot, determine the value of the Michaelis constant. [S]/ (10-6 mol dm-3) v/ (10-3 mol dm-3 s-1) 1.00 2.00 3.00 4.00 16.7 33.3 41.1 49.8
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You are working on an enzyme that obeys standard Michaelis-Menten kinetics. What variable is the V, dependant on if the concentration of the substrate is substantially higher than the concentration of the enzyme? [S] [E] [ES] O [P] O not enough information provided
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For an enzyme-catalyzed reaction, the velocity was determined at two different concentrations of the substrate. Estimate the value of Vmax. [S] (MM) 10 20 O 88 nmol/min 79 nmol/min 67 nmol/min V(nmol/min) 51 nmol/min 27 Not enough information is given to form a reasonable estimation. 48