The Ki for a competitive inhibitor pyrrole-2-carboxylate is 0.05M. Calculate the initial velocity if the substrate concentration is 0.25M and 1.0 mole/liter of the inhibitor is added to the reaction
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
The Ki for a competitive inhibitor pyrrole-2-carboxylate is 0.05M.
Calculate the initial velocity if the substrate concentration is 0.25M and 1.0 mole/liter of the inhibitor is added to the
reaction.
A form of enzyme inhibitor known as a competitive inhibitor pits the substrate against the enzyme's active site for binding. Its structure is similar to that of the substrate, and it has the ability to momentarily obstruct the active site, lowering the enzyme's capacity to bind and transform the substrate. This inhibition can be removed by raising the concentration of the substrate.
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