If Vmax for a reaction is 10 μM ⋅ s-1 and the KM  is 0.5 μM, what is the reaction velocity when the substrate concentration is 2 μM?

Q: Consider a uniport system where a carrier protein transports an uncharged substance A across a cell…

A: ∆G = R T ln (Ainside /Aoutside)  Where G is free energy change for the transport of uncharged…

Q: An enzyme catalyzes a reaction at a velocity of 20 micromole/min when the concentration of substrate…

A: The Michaelis-Menten equation represents the relationship between substrate concentration and…

Q: You are trying to come up with a drug to inhibit the activity of an enzyme  thought to have a role…

A: In competitive inhibition, an inhibitor molecule interferes with the binding of normal substrate to…

Q: What would the activity of a lysozyme sample be if it was able to change the absorbance of a cell…

A: It is asked what would be the activity of lysozyme sample if it was able to change the absorbance of…

Q: Calculate the rate enhancement for the enzyme pair: Enzyme 1: Kcat (sec-1)= 5.5 KM (µ M) = 1245…

A: Enzymes are known to enhance (increase) the rate of a reaction, compared to the rate at which the…

Q: The turnover number for an enzyme that approximates Michaelis-Menten kinetics is known to be 500…

A: Turn over number of an enzyme is the maximum number of substrate molecules that are converted into…

Q: 1. Calculate Vmax and Km???

A: Enzymes are usually a protein molecules that catalyzes the biochemical reactions without being…

Q: [S] mM 20 50 100 200 300 500 700 Initial Velocity (mM/min) 1.012 1.950 2.823 3.637 4.024 4.398 4.580

A: Enzyme kinetics can  be determined by constructing mm plot or lb plot. With them km and Vmax can be…

Q: rformed a lactate dehydrogenase (LDH) assay multiple times e same. This graph shows the results…

A: An enzyme is a protein which is required for carrying out a particular chemical reaction inside the…

Q: Glycogen provides short term energy storage primarily in liver and muscle.  Defects in the enzymes…

A: NOTE: The blanks are numbered as 1, 2, 3 and 4.  The blood glucose levels is the measure of…

Q: Án enzyme that follows Michaelis-Menten kinetics has a KM value of 3.00 µM and a kat value of 181s1.…

A: An enzyme is a type of biological catalyst that aids in the acceleration of chemical reactions.…

Q: s) In two experiments carried out in a research lab, an enzyme reaction is run with and without an…

A: Enzymes are proteins that catalyze biochemical reactions. They contain an active site where the…

Q: In the hydrolysis (saponification) of ethyl acetate, a plot of In(k,-k) against time is a straight…

A: To determine the reaction rate constant  k  for the hydrolysis of ethyl acetate, we need to use the…

Q: The diffusivity of amino acids in polyacrylamide gel is approximately 1x10^-9 cm2./s calculate the…

A: Introduction  Electrophoresis is a technique used to separate and analyze charged particles, such as…

Q: Cooperativity yields a sigmoidal kinetic curve. Which of the following is TRUE?

A: Co-operativity can be described as a phenomenon where the enzyme shape changes with the substrate…

Q: Bradford Assay-Standard Curve 0.800 0.700 0.600 0.500 0.400 0.300 y = 0.0148x - 0.003 R? = 0.9981…

A: Spectrophotometric analysis can be carried out to find the concentration (or amount) of protein in a…

Q: Calculate KI (in units of nmol L-1) for a competitive inhibitor that has α = 1.65 when 11.0 nmol L-1…

A: α=1+​ [I]​/KIWhere:α (alpha) is the degree of inhibition[I] is the concentration of the inhibitorKI…

Q: In your acid phosphatase enzyme kinetics lab, you constructed a Lineweaver Burk plot. Lefs assume…

A: Enzymes are biological catalysts that help chemical processes occur faster. Enzymes are proteins…

Q: What is the concentration of enzyme (in mM) needed to achieve a Vmax of 8.00 mM/s if the enzyme has…

A:

Q: calculate the reaction velocity at saturating substrate concentrations. Your numerical answer is…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

Q: 1/v (sec/μmol) Enzymes A and B catalyze different reactions but use the same reactant molecule as a…

A: The Km and Vmax are two parameters we are looking into if we study enzymes. Therefore, we can use…

Q: Calculate the Km of the above enzyme.

A: This is the concept of enzyme-catalyzed reaction which is given by Michaelis-Menten. The equation…

Q: From a kinetics experiment, Kcat was determined to be 55sec^-1. For the kinetic assay, 0.05mL of a…

A: The concentration of enzyme stock solution was 0.05mg/ml. i.e. 1ml contain 0.05mg of enzyme. We took…

Q: The value of Vmax for an enzyme-catalyzed reaction: A) most often reflects the chemical steps of…

A: The correct answer is:A) most often reflects the chemical steps of catalysisHere's why:Vmax (maximum…

Q: Given the line-weaver Burke plot below for Enzyme Y, identify the Vmax for Enzyme Y. -10 30 1/v…

A: 0.056Explanation:To find the Vmax​ from a Lineweaver-Burk plot, we need to take the reciprocal of…

Q: Why is the ½ Vmax of a reaction commonly used to determine the efficiency the reaction, instead of…

A: The answer to the problem is the first option It is impossible to know if the reaction has reached…

Q: Mike has determined that enzyme he is attempting to purify has an isoelectric point of 4.5 (pI =…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

Q: The following data were collected for an enzyme that can be described by Michaelis Menton kinetics.…

A: In-order to get accurate Km and Vmax values, we need to draw the LB plot. The LB plot has 1V (i.e.…

Q: At steady state,[Es} stays the same although substrate concentraion is steadily decreasing and…

A: According to steady-state assumption, the concentration of [ES] (Enzyme –substrate) complex is…

Q: At what substrate concentration would an enzyme with a kcat of 33.0 s-1 and a Km of 0.0046 M operate…

A: The Michali s-Menten kinetics is enzyme kinetics that is common in biochemistry to detect and…

Q: what concentration of a competitive inhibitor is required to yield 60% inhibition of an enzyme at a…

A: For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…

Q: at what substrate concentration is v= 5.0 mM s^-1 for the enzyme catalyzed hydrolysis of trehalose?

A: 1 molecule trehalose is hydrolyzed into 2 glucose molecules by the trehalase enzyme.

Q: purified protein sample was used in a reaction, resulting in an activity of 696.7 nmol min-1. The…

A: The activity of protein sample refers to the quantity of active protein present in the purified…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.