1. Calculate Vmax and Km???
Chapter21: Heparin Infusion Calculations
Section: Chapter Questions
Problem 1.1P
Related questions
Question
![Substrate
Initial velocity Vo
concentration [S] µM s-1
10
0.13
25
0.27
50
0.45
100
0.65
150
0.77
200
0.85
300
0.94
500
1.03
1. Calculate Vmax and Km???
The enzyme is dissolved at a concentration of 1
mg/ml and has a molecular weight of 50 kDa.
a) What is the turnover number of the enzyme?
b) What is the specificity constant of the
enzyme?
c) Is the enzyme operating near the maximum
rate possible, i.e. is the enzyme diffusion
controlled?
please show all working and formulas as I am
trying to learn. thank you](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ffe97b099-d6ed-461e-99fb-0b0ad0d97cf4%2F1a7f26a8-57dc-425b-abe9-10fe9e84cf01%2Fgpm68un_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Substrate
Initial velocity Vo
concentration [S] µM s-1
10
0.13
25
0.27
50
0.45
100
0.65
150
0.77
200
0.85
300
0.94
500
1.03
1. Calculate Vmax and Km???
The enzyme is dissolved at a concentration of 1
mg/ml and has a molecular weight of 50 kDa.
a) What is the turnover number of the enzyme?
b) What is the specificity constant of the
enzyme?
c) Is the enzyme operating near the maximum
rate possible, i.e. is the enzyme diffusion
controlled?
please show all working and formulas as I am
trying to learn. thank you
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