You are asked to prepare HEPES buffered saline with the following constituents: НЕРES 10mM KCI 5mM NaCI 135mM CaCl2 1mM M9SO4 0.6mM Glucose 6mM You have previously made up 100 mM stock solutions of HEPES, KCI, CaCl2, and M9SO4 and a 1M stock of NaCl. The MW of glucose is 180.16 g/mol What volume of HEPES would you require to make up 400ml of buffer?

You are asked to prepare HEPES buffered saline with the following constituents: НЕРES 10mM KCI 5mM NaCI 135mM CaCl2 1mM M9SO4 0.6mM Glucose 6mM You have previously made up 100 mM stock solutions of HEPES, KCI, CaCl2, and M9SO4 and a 1M stock of NaCl. The MW of glucose is 180.16 g/mol What volume of HEPES would you require to make up 400ml of buffer?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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