I II II Methanol (mM) Rate of Reaction (mMmin-1) Rate of Reaction in presence of 5.0 µM Ethanol 0.50 23.5 16.67 1.0 32.2 25.25 1.5 36.9 30.49 2.5 - 41.8 37.04 3.5 44.0 38.91 (Nelson, 2008)

Biochemistry
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ISBN:9781319114671
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Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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11) Using the data above, construct Lineweaver-Burk plots. First, graph the inverse of the reaction velocity (rate of reaction) data in column II versus the inverse of the methanol concentration in column I. Then, on the same graph, plot the inverse of the reaction velocity (rate of reaction) data in column III versus the inverse of the methanol concentration in column I

 

**Table: Effect of Methanol Concentration on Reaction Rate**

| Methanol (mM) | Rate of Reaction (mM min⁻¹) | Rate of Reaction in Presence of 5.0 μM Ethanol |
|------------------|--------------------------------|-----------------------------------------------------|
| 0.50                | 23.5                                        | 16.67                                                    |
| 1.0                  | 32.2                                        | 25.25                                                    |
| 1.5                  | 36.9                                        | 30.49                                                    |
| 2.5                  | 41.8                                        | 37.04                                                    |
| 3.5                  | 44.0                                        | 38.91                                                    |

**Description:**

This table represents the rates of a chemical reaction at different methanol concentrations. Column I lists methanol concentrations in millimoles (mM). Column II shows the corresponding rates of reaction without ethanol. Column III indicates the rates of reaction when 5.0 μM of ethanol is present.

*Reference: Nelson, 2008*
Transcribed Image Text:**Table: Effect of Methanol Concentration on Reaction Rate** | Methanol (mM) | Rate of Reaction (mM min⁻¹) | Rate of Reaction in Presence of 5.0 μM Ethanol | |------------------|--------------------------------|-----------------------------------------------------| | 0.50 | 23.5 | 16.67 | | 1.0 | 32.2 | 25.25 | | 1.5 | 36.9 | 30.49 | | 2.5 | 41.8 | 37.04 | | 3.5 | 44.0 | 38.91 | **Description:** This table represents the rates of a chemical reaction at different methanol concentrations. Column I lists methanol concentrations in millimoles (mM). Column II shows the corresponding rates of reaction without ethanol. Column III indicates the rates of reaction when 5.0 μM of ethanol is present. *Reference: Nelson, 2008*
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