Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
Please help me answer and explain these questions:
1. Compare the abundance of A280 chromophores (W, Y, and C) to Bradford sites (loosely R and K) in BSA.
2. We used a protocol that corrects the path length in each well and normalizes the absorbance to the value that would be obtained if the path length were 1 cm (the standard path length). Using this observation and your A280 calibration data, what is your measured e280, for BSA?
Expert Solution
Step 1
Answer :
Q1. Advantage absorbance at 280 nm : It is very simple. Also the sample is not destroyed
Disadvantage is of interference from nonprotein chromophores.
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