1. Compare the abundance of A280 chromophores (W, Y, and C) to Bradford sites (loosely R and K) in BSA.

Human Anatomy & Physiology (11th Edition)
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Please help me answer and explain these questions:

1. Compare the abundance of A280 chromophores (W, Y, and C) to Bradford sites (loosely R and K) in BSA.

2. We used a protocol that corrects the path length in each well and normalizes the absorbance to the value that would be obtained if the path length were 1 cm (the standard path length). Using this observation and your A280 calibration data, what is your measured e280, for BSA?

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Q1. Advantage absorbance at 280 nm : It is very simple. Also the sample is not destroyed

Disadvantage is of interference from nonprotein chromophores.

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