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What is the effect of salinity on the rates of photosynthesis versus respiration in Euglena gracilis as shown in Figure 2? What does the difference in the initial slopes of the curves in the left panel tell us?

What is the effect of salinity on the rates of photosynthesis versus respiration in Euglena gracilis as shown in Figure 2? What does the difference in the initial slopes of the curves in the left panel tell us?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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What is the effect of salinity on the rates of photosynthesis versus respiration in Euglena gracilis as shown in Figure 2? What does the difference in the initial slopes of the curves in the left panel tell us?

Case Study 2: The researchers used stock cultures of wild-type Euglena gracilis. (a unicellular, freshwater alga). Cells were incubated in the dark in 1.2 mL of 0.1 M potassium phosphate (pH 7.4), for determination of oxygen uptake. After approximately 50% of dissolved oxygen had been consumed, a light stimulus was applied to the chamber for determination of oxygen production using a Clark-type oxygen electrode. An aliquot of 50 µL of cell suspension was extracted with 80% acetone and the amounts of Chlorophylls a and b were determined spectrophotometrically. 80 40 a 200 E 60 30 100 40 20 오100 20 200 250 500 800 1000 250 500 800 1000 Light intensity (umol quanta m-2 s Light intensity (umol quanta m-2 s-1 Figure 2: Effect of photon flux density (light intensity) on the rate of oxygen production of Euglena gracilis. The numbers shown on the right side of the graph represent the concentration of NaCl (mM) added to the growth medium (● = 0 NaCl, O = 100 mM NaCl, A = 200 mM NaCl). The cells were exposed to the indicated light intensities through density filters and the rate of oxygen production was measured at 30°C after 2 cycles of respiration/photosynthesis (left panel). Thereafter, 10 µM DCMU was added and the rate of respiration was measured (right panel). The data shown represent the mean + standard error of 3 different cultures assayed. In the right panel, a: P<0.05 (200 vs. 0 NaCl)
Transcribed Image Text:Case Study 2: The researchers used stock cultures of wild-type Euglena gracilis. (a unicellular, freshwater alga). Cells were incubated in the dark in 1.2 mL of 0.1 M potassium phosphate (pH 7.4), for determination of oxygen uptake. After approximately 50% of dissolved oxygen had been consumed, a light stimulus was applied to the chamber for determination of oxygen production using a Clark-type oxygen electrode. An aliquot of 50 µL of cell suspension was extracted with 80% acetone and the amounts of Chlorophylls a and b were determined spectrophotometrically. 80 40 a 200 E 60 30 100 40 20 오100 20 200 250 500 800 1000 250 500 800 1000 Light intensity (umol quanta m-2 s Light intensity (umol quanta m-2 s-1 Figure 2: Effect of photon flux density (light intensity) on the rate of oxygen production of Euglena gracilis. The numbers shown on the right side of the graph represent the concentration of NaCl (mM) added to the growth medium (● = 0 NaCl, O = 100 mM NaCl, A = 200 mM NaCl). The cells were exposed to the indicated light intensities through density filters and the rate of oxygen production was measured at 30°C after 2 cycles of respiration/photosynthesis (left panel). Thereafter, 10 µM DCMU was added and the rate of respiration was measured (right panel). The data shown represent the mean + standard error of 3 different cultures assayed. In the right panel, a: P<0.05 (200 vs. 0 NaCl)
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