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Cell lysis and protein purification of GST-DHFR-His using T7 RNA polymerase protein to express DHFR Protein dihydrofolate reductase (DHFR) IMAC chromatography to purify DHFR and GST-tag Eukaryotic cell → Cell culture SDS-page analysis using gel electrophoresis of purified GST- DHFR-His and cell lysate HEK293T cells overexpressing Myc-Flagged Determined the Western blot analysis with the transfer of proteins to Protein quantification by Coomassie Assay concentration of the unknown sample (14.7mg/L) -- -- a nitrocellulose filter tagged DHFR Sequence analysis of tagged DHFR fusion proteins Tag is in the C- terminal of DHFR GST-tag added to Six-histidine the N-terminus to added to the increase solubility DHFR C-terminus protein and is GST- tag

Cell lysis and protein purification of GST-DHFR-His using T7 RNA polymerase protein to express DHFR Protein dihydrofolate reductase (DHFR) IMAC chromatography to purify DHFR and GST-tag Eukaryotic cell → Cell culture SDS-page analysis using gel electrophoresis of purified GST- DHFR-His and cell lysate HEK293T cells overexpressing Myc-Flagged Determined the Western blot analysis with the transfer of proteins to Protein quantification by Coomassie Assay concentration of the unknown sample (14.7mg/L) -- -- a nitrocellulose filter tagged DHFR Sequence analysis of tagged DHFR fusion proteins Tag is in the C- terminal of DHFR GST-tag added to Six-histidine the N-terminus to added to the increase solubility DHFR C-terminus protein and is GST- tag

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Cell lysis and protein purification of GST-DHFR-His using T7 RNA polymerase protein to express DHFR Protein dihydrofolate reductase (DHFR) IMAC chromatography to purify DHFR and GST-tag Eukaryotic cell → Cell culture SDS-page analysis using gel electrophoresis of purified GST- DHFR-His and cell lysate HEK293T cells overexpressing Myc-Flagged tagged DHFR Determined the Western blot analysis with the transfer of proteins to Protein quantification by Coomassie Assay concentration of the a nitrocellulose filter unknown sample (14.7mg/L) Sequence analysis of tagged DHFR fusion proteins GST-tag added to the N-terminus to Tag is in the C- terminal of DHFR Six-histidine added to the increase solubility DHFR C-terminus protein and is GST- tag
Transcribed Image Text:Cell lysis and protein purification of GST-DHFR-His using T7 RNA polymerase protein to express DHFR Protein dihydrofolate reductase (DHFR) IMAC chromatography to purify DHFR and GST-tag Eukaryotic cell → Cell culture SDS-page analysis using gel electrophoresis of purified GST- DHFR-His and cell lysate HEK293T cells overexpressing Myc-Flagged tagged DHFR Determined the Western blot analysis with the transfer of proteins to Protein quantification by Coomassie Assay concentration of the a nitrocellulose filter unknown sample (14.7mg/L) Sequence analysis of tagged DHFR fusion proteins GST-tag added to the N-terminus to Tag is in the C- terminal of DHFR Six-histidine added to the increase solubility DHFR C-terminus protein and is GST- tag
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