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A set of DSC curves for a solution of Lysozyme at several pH values is given in the image.  A. Explain how the curves above relate to the enthalpy of unfolding for each of these curves.

A set of DSC curves for a solution of Lysozyme at several pH values is given in the image.  A. Explain how the curves above relate to the enthalpy of unfolding for each of these curves.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A set of DSC curves for a solution of Lysozyme at several pH values is given in the image. 

A. Explain how the curves above relate to the enthalpy of unfolding for each of these curves. 

B. Question b is attached as an image.

8 pH 4.5 рH 3.0 6 pH 2.0 2 20 30 40 50 60 70 80 90 T/°C Cp ipr / JK-1.g-1
Transcribed Image Text:8 pH 4.5 рH 3.0 6 pH 2.0 2 20 30 40 50 60 70 80 90 T/°C Cp ipr / JK-1.g-1
b. Accurate measurement of AC, sufficiently good to determine the heat capacity change, an alternative approach is to perturb the stability using pH and measure enthalpies at several different melting temperatures. At pH 4.5, you determine AHº to be 140 kcal mol. At pH 2.0, you measure a value for AH of 122 kcal moll. Estimate AC, of unfolding for lysozyme. You may assume that the can be difficult using DSC. If the baselines are not difference in pH makes no contribution to the thermodynamics of unfolding. This is not
Transcribed Image Text:b. Accurate measurement of AC, sufficiently good to determine the heat capacity change, an alternative approach is to perturb the stability using pH and measure enthalpies at several different melting temperatures. At pH 4.5, you determine AHº to be 140 kcal mol. At pH 2.0, you measure a value for AH of 122 kcal moll. Estimate AC, of unfolding for lysozyme. You may assume that the can be difficult using DSC. If the baselines are not difference in pH makes no contribution to the thermodynamics of unfolding. This is not
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