In an experiment to test the activity of lactase under different conditions, tubes containing buffer, ONPG, and lactase were set up. Absorbance was recorded from the spectrophotometer. Select true or false for each of the statements regarding the lactase experiment below! v The absorbance will decrease at a constant rate as the amount of A. TRUE ONPG is increased. B. FALSE v As pH increases above 1, there would be an initial increase in intensity of yellow color which then plateaus. v Greater amount of lactase is released when experiment is performed at 37°C than at 100°C. v Adding increasing amount of lactose would cause the absorbance to decrease.

Human Anatomy & Physiology (11th Edition)
11th Edition
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Author:Elaine N. Marieb, Katja N. Hoehn
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In an experiment to test the activity of lactase under different conditions, tubes containing buffer, ONPG, and lactase were set up. Absorbance was
recorded from the spectrophotometer.
Select true or false for each of the statements regarding the lactase experiment below!
v The absorbance will decrease at a constant rate as the amount of
A. TRUE
ONPG is increased.
B. FALSE
v As pH increases above 1, there would be an initial increase in
intensity of yellow color which then plateaus.
- v Greater amount of lactase is released when experiment
is performed at 37°C than at 100°C.
v Adding increasing amount of lactose would cause the absorbance to
decrease.
Show All
c.docx.pdf
4600885-Orde..docx
F4
del
2$
4.
8
9.
7
5
3
Y
R
K
6
Transcribed Image Text:In an experiment to test the activity of lactase under different conditions, tubes containing buffer, ONPG, and lactase were set up. Absorbance was recorded from the spectrophotometer. Select true or false for each of the statements regarding the lactase experiment below! v The absorbance will decrease at a constant rate as the amount of A. TRUE ONPG is increased. B. FALSE v As pH increases above 1, there would be an initial increase in intensity of yellow color which then plateaus. - v Greater amount of lactase is released when experiment is performed at 37°C than at 100°C. v Adding increasing amount of lactose would cause the absorbance to decrease. Show All c.docx.pdf 4600885-Orde..docx F4 del 2$ 4. 8 9. 7 5 3 Y R K 6
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