Q1) What is the name of the enzyme found in potato extract? Answer: Catechol Q2) What is the substrate? Answer: THO Q3) Name of product of this enzyme catalyzed reaction? Answer: Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1? Catechol Benzoquinone
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
 alt="Table 1 - Comparison of the effect of catechol concentration on the amount of product formed.
Absorbance
Potato
extract
Absorbance
0 mins
after 30mins (2nd reading)
(mL)
1st reading
1
Tube #
la
blank
2a
3a
4a
1
1
1
dH₂O Catechol
(mL) (mL)
7
5
3
1
0
2
4
6
0.00
0.060
0.033
0-05-2
Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1?
Time for reading: 3:21
-0.11
Absorbance:
Time for reading: 3.36
Q5) Tube la serves as a control, but why is this control needed?
Absorbance:
0.197
Time for reading: 3.37
Based on the data from Table 1 answer these questions:
Q1) What is the name of the enzyme found in potato extract? Answer:
catechol
Q2) What is the substrate? Answer:
THO
Q3) Name of product of this enzyme catalyzed reaction? Answer:
Absorbance: 0.152
Time for reading: 3:39
Absorbance: . 166
ness
Catechol
Benzoquinone
Subtract 1st
from 2nd
reading
-0.01
0-137
0.11.19
0.119
Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different at
the 2nd absorbance reading? Explain why - hint you need to mention enzyme in your answer.
Q7) What is the relationship between catechol concentration and amount of benzoquinone formed?
column's data do you use for this interpretation?
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