You have two different enzymes, each with 2 substrates, S1 and S2, and 2 products, P1 and P2. A. Write an ordered sequential kinetic mechanism for Enzyme A, using Cleland terminology. B. Write a ping pong kinetic mechanism for Enzyme B, using Cleland terminology.
You have two different enzymes, each with 2 substrates, S1 and S2, and 2 products, P1 and P2. A. Write an ordered sequential kinetic mechanism for Enzyme A, using Cleland terminology. B. Write a ping pong kinetic mechanism for Enzyme B, using Cleland terminology.
Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Can you please help show how to use the cleland terminology for these two question?
Transcribed Image Text:You have two different enzymes, each with 2 substrates, S1 and S2, and 2 products, P1 and P2.
A. Write an ordered sequential kinetic mechanism for Enzyme A, using Cleland terminology.
B. Write a ping pong kinetic mechanism for Enzyme B, using Cleland terminology.
Expert Solution
Mechanisms of multi-substrate enzyme catalyzed reactions
There are three major reaction mechanisms seen with enzymes that catalyze a reaction involving 2 substrates and results in the release of 2 products. They are;
- Ordered Sequential
- Random Sequential
- Ping Pong or Double-Displacement reaction
Here, the substrates are S1 & S2 , while the products formed are P1 and P2. We will represent the enzyme as 'E'.
The Cleland terminology is a way to represent these reaction mechanisms using lines and arrows. It is read from left to right.
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