A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography.When subjected to gel electrophoresis in the presence of sodium dodecyl sulfate (SDS), the protein givesthree bands with molecular masses of 180, 160, and 60 kDa. When electrophoresis is carried out in thepresence of SDS and dithiothreitol, three bands are again formed, this time with molecular masses of160, 90, and 60 kDa. Determine the subunit composition of the protein and clearly explain how youcame to this determination.

Size exclusion chromatography is a technique which separates molecules according to their size…

Answered: A protein has a molecular mass of 400…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

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