The pH vs charge graph for a triprotic amino acid is shown below. Please answer the followingquestions about the amino acid.Net Charge024Alpha amino [Select]6R [Select]pHa. Which of the following triprotic amino acids is this?Alpha carboxyl [Select]81012[ Select]1410.50-0.5-1b. What is the isoelectric point? (Please select the appropriate range in which the isoelectric pointfalls) (Hint - look up the pKa values in the pKa table for amino acids, which was in video one oftoday's lecture) [Select]-1.5c. What form of this amino acid dominates at a pH of 14? Please select the correct form for eachionizable group.-2

An amino acid with the ability to donate 3 protons (3 H+) is called a triprotic amino acid. The 3…

Answered: The pH vs charge graph for a triprotic…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The diprotic amino acid proline is shown below as it would appear in a vacuum. The pKa of the alpha carboxyl group is 1.95 and the pKa of the alpha amino group is 10.64. HN- C C. HO, | | CH2 CH2 CH2 a. What is isoelectric point? (Round your answer to two decimal places, for example: 0.13 or 1.45 or 3.30). b. What is the average charge of the amino acid at a pH of 10? Please round your answer to the nearest two decimal places. (Please make sure to include + or - in front of your response. Even if the answer is positive you need to include a plus sign to indicate a positive charge. Please do not add a space between the + or - symbol and your numerical answer. For example: +0.13 or -1.45 or -3.30).
Draw the amino acid below in its most protonated form, any intermediately protonated forms, and its least protonated form. Also draw a titration curve for this amino acid, with pH on the y-axis and equivalents on the x-axis going from acidic to basic (most protonated to least protonated) using the pka values below. Match the protonated/deprotonated forms to the appropriate regions of the titration curve. Scan your drawing, and upload as a pdf, jpeg, or tiff file. H3C O NH₂ pKas are provided below: OH
Describe the amino acid illustrated above (at pH = 7.0). (the following amino acid at pH = 7 (aqueous form): (COO-)–CH(NH3+)–CH2–(COO-)) it is a non-polar, positively-charged amino acid it is a polar, positively-charged amino acid it is a polar, uncharged amino acid it is a polar, negatively-charged amino acid it is a non-polar, negatively-charged amino acid
Consider a protein in which a negatively charged glutamic acid side chain (pKa=4.2) makes a salt bridge (ion-ion interaction) with a positively charged histidine side chain (pKa=6.5). Part C: Justify your answer with calculations of partial charges on these amino acid side chains at pH=7.9.(Hint: Consider lessons from Coulomb's law, and the Henderson-Hasselbalch equation.)
None
The aromatic side chain of Trp residues in proteins and peptides can be chemically modified by two photochemical reactions using ultraviolet light (hv) at 20°C (Figure 1). hv CCI3CO₂H (10 mm), Buffer A (pH 8.0) O HO HN HN- O A. Acidic NH ww O B. Basic O c. Hydrophobic O D. Polar neutral OB. Indole NH ww Figure 1 Photochemical reactions of Trp side chain with CHCl3 and CCI₂CO₂H O A. Imidazole Each substitution on Trp replaces one hydrogen atom attached to a carbon somewhere on the indole ring. Photochemical modification percentages of various Trp residues in carbonic anhydrase at 20°C are shown in Table 1. Table 1 Photochemically Modified in Carbonic Anhydrase Reactant Buffer W4 W15 W96 W122 W190 W207 W243 51% 0% 44% A 37% 0% 0% CHCI 3 0% CHCI₂ B 0% 0% 0% 0% 0% 0% 55% CCI,CO₂H A 72% 66% 0% 100% 100% 0% 75% O c. Pyrimidine OD. Pyrrole hv W15 and W96, which are in the interior of carbonic anhydrase, are 21% and 29% modified respectively by 10 mM CHCl3 in Buffer A at 75°C, a temperature…
Indicate the net charge of cysteine at pH 9.5 Express your answer using two significant figures.
What average positive charge of an amino acid side chain with pKa 10.9 at a pH value of 11.9? Answer up to two decimals. (Please type answer no write by hend)
Describe the important biochemical properties of carbohydrates and why carbohydrates would be considered polar molecules. A▾ B I X @ F 7
1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL with water (i.e. 0.1 mL of the solution was added to 3.9 mL of water). 2 mL of this solution was then mixed with 18 mL of water. What is the concentration of the diluted protein solution? Space to show your workings:
a. what general category would you place this molecule in of the four categories of biomolecules? b. List 5 functional groups you see in this molecule?
The amino acid arginine ionizes according to the following scheme: (a) Calculate the isoelectric point of arginine. You can neglect contributions from form I. Why? (b) Calculate the average charge on arginine when pH = 9.20. (c) Is the value of average charge you calculated in part b reasonable, given the pI you calculated in part a? Explain your answer

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