A small peptide has two pKa values of 3.42 and 8.74. What is the isoelectric point for this peptide? Report your answer as a number below to three significant figures
A small peptide has two pKa values of 3.42 and 8.74. What is the isoelectric point for this peptide? Report your answer as a number below to three significant figures
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A small peptide has two pKa values of 3.42 and 8.74. What is the isoelectric point for this peptide? Report your answer as a number below to three significant figures
Expert Solution
Step 1
Isoelectric point: The isoelectric point(pI) is the pH at which a particular molecule carries no net electrical charge. The net charge on the molecule is affected by the pH of its surrounding environment. It is the mean of Pka values for a molecule, Mathematically. The pKa of each amino component amino acid determines the pI of a protein.
pI = 1/2 (pKa1 + pKa2)
pI = 1/2 (3.42 + 8.74 )
pI = 1/2 (12.16)
pI = 7.79
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