a. What is isoelectric point? (Round your answer to two decimal places, for example: 0.13 or 1.45 or 3.30).

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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The diprotic amino acid proline is shown below as it would appear in a vacuum. The pKa of the alpha
carboxyl group is 1.95 and the pKa of the alpha amino group is 10.64.
HN- C
C.
HO,
|
|
CH2
CH2
CH2
a. What is isoelectric point? (Round your answer to two decimal places, for example: 0.13 or 1.45 or
3.30).
b. What is the average charge of the amino acid at a pH of 10? Please round your answer to the
nearest two decimal places. (Please make sure to include + or - in front of your response. Even if the
answer is positive you need to include a plus sign to indicate a positive charge. Please do not add a
space between the + or - symbol and your numerical answer. For example: +0.13 or -1.45 or -3.30).
Transcribed Image Text:The diprotic amino acid proline is shown below as it would appear in a vacuum. The pKa of the alpha carboxyl group is 1.95 and the pKa of the alpha amino group is 10.64. HN- C C. HO, | | CH2 CH2 CH2 a. What is isoelectric point? (Round your answer to two decimal places, for example: 0.13 or 1.45 or 3.30). b. What is the average charge of the amino acid at a pH of 10? Please round your answer to the nearest two decimal places. (Please make sure to include + or - in front of your response. Even if the answer is positive you need to include a plus sign to indicate a positive charge. Please do not add a space between the + or - symbol and your numerical answer. For example: +0.13 or -1.45 or -3.30).
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