(a) 1Normalized fluorescence0.80.60.40.205055OM0.100 M0.200 M0.300 M0.500 M1.00 M2.00 M6011358865Temp. (°C)707580Where is fully folded protein?• Where is fully unfolded protein?• Where is partially folded protein?• To what does SYPRO orange bind?• Why does fluorescence increase as a function oftemperature?● Define a melting temperature for a protein.• Demonstrate how an estimated meltingtemperature of the protein in zero molar ligandcan be determined.• What is the effect of increasing the molarconcentration on melting temperature for thisprotein?• Why is melting temperature a useful measurementto make for a protein especially if youare interested in protein aggregation?

There are four classes of biological macromolecules - proteins, nucleic acid, lipids and…

Answered: (a) 1 Normalized fluorescence 0.8 0.6…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Two proteins bind the same ligand, and the data is shown below. What is the KD value for Protein B? 0.5 nM 8 nM correct answer not given 1.8 nM
Make a table with a scale of absorbance and the concentration of protein in Chromatin sample from the following data for excel graph Absorbance=660nm following data are of tubes with concern A =0 B=0.036 C=0.011 D=0.001 E=0.027 F=0.020 G=0.032 H1=0.176 H2=0.183 I1=0.150 I2=0.171 also plot the graph??
You perform electrophoresis of 3 proteins, Hemoglobin (pl: 6.8), DNA Polymerase (pl: 5.2) and Porcine pepsin (pl: 1.0) in a Tris-Glycine buffer (pH 5.2). (pl = Isoelectric point). Select which of the following is true. O All proteins will migrate at the same rate O All proteins will migrate at the same direction Porcine pepsin will migrate toward the electrode Porcine pepsin will not migrate and stay in the well
6. Consider the following proteins to answer the questions below: Protein Size (kDa) ε at 280 nm 10 7000 14000 3000 50000 A B C D 50 10 50 pl 4 4 8 8 Red Colored? Yes No No No a. Sketch the UV-Vis spectrum of each pure protein (A, B, C, and D) from 240- 480 nm if they were all present at 20 µM. Note: I want four clearly labeled spectra on one plot.
Draw a UV-Vis spectrum for each protein (shown in the table below) (A, B, C, and D) from 240 – 480 nm if they were all present at 20 µM.
6. Consider the following proteins to answer the questions below: Protein Size (kDa) pl ε at 280 nm 10 4 7000 50 4 14000 10 8 3000 50 8 50000 A B C C Red Colored? Yes No No No b. Describe a two-step purification procedure that could be used to purify/isolate protein A from the other proteins. In your response, describe the type of chromatography used, the pH of buffer needed, and a labeled chromatogram (include absorbances at both 280 and 400 nm). Make sure you note which "fraction/sample" is needed from the first step to proceed/use for the second step. Use another page if necessary.
Onion Cell Plasmolysis For comparison, the following picture is of onion cells in pure water. Identify the structures listed below. A. B. K C. D. Identify the structure labeled A (thick line Identify the structure labeled B ✓ [Choose ] Cell membrane Nucleus Cytoplasm Cell wall Identify the structure labeled C [Choose ] Identify the structure labeled D (thin line [Choose ] between the white and purple))
7. [10'] For a bacteriophage 77, the following data at zero concentration have been obtained: Sedimentation coefficient: $20,w = 453 S. Diffusion coefficient: D₂0. = 6.03×10-8 cm² s¹¹. 20,w Specific volume V20 = 0.639 cm³ g¹ Calculate the molecular mass M of the bacteriophage.
A sample of a protein is analyzed by CE using a neutral-coated capillary with a total length of 25.0 cm and a distance to the detector of 22.0 cm. Using an applied voltage of 20.0 kV, the protein was detected at a migration time of 16.2 min. The diffusion coefficient for the protein is 2.4 x 10-7 cm?/s. (a) Calculate the apparent electrophoretic mobility of the protein. (b) Calculate the expected number of theoretical plates for the analysis.
1- Describe any errors in your technique. How could you improve upon your technique in order to get a better and more accurate standard curve graph? 2-Estimate (from your data) the [Protein] of your unknown (0.103) based on comparing to the standards. Include the correct unit. This estimate should be an integer (no decimals).
What is the pelleting time for eukaryotic ribosome subunits, 40S and 60S, in a rotor with k = 180? Assume that the medium viscosity and density are similar to water at %3D 20°C.
A spherical cell with the diameter of 10uMhas a protein concentration of 20 mg/ml. Determine the number of protein molecules within the cell if the molecular weight of an average protein is 50,000 daltons (g/mol). Recall that Avogadro's number is N₁ = 6.0221367×1023 molecules/mol.

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