You are running a 500L Protein A purification column to purify a 150,000 Da monoclonal antibody. Based on data from previous experiments, you know that the association constant between Protein A and this monoclonal antibody is 64 and that the dynamic binding capacity of the column is approximately 0.33 m.M. Historical experience with the column indicates that the elution step is only 85% efficient. If, after loading the column, you measure a concentration of monoclonal antibody in the mobile phase to be 0.02, how much monoclonal antibody (in kg) should you expect to M elute from the column?
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
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