Draw a UV-Vis spectrum for each protein (shown in the table below) (A, B, C, and D) from 240 – 480 nm if they were all present at 20 µM. ProteinABCDSize (kDa)10501050pl4488ε at 280 nm700014000300050000Red Colored?YesNoNoNo

Answered: Draw a UV-Vis spectrum for each protein…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Draw a UV-Vis spectrum for each protein (shown in the table below) (A, B, C, and D) from 240 – 480 nm if they were all present at 20 µM.

Protein
A
B
C
D
Size (kDa)
10
50
10
50
pl
4
4
8
8
ε at 280 nm
7000
14000
3000
50000
Red Colored?
Yes
No
No
No

Expert Solution

Step 1

UV-Vis spectroscopy is a technique that measures the absorption or transmission of ultraviolet and visible light by a sample. The spectrum obtained is called a UV-Vis spectrum.

The UV region of the spectrum typically ranges from 200 to 400 nm, while the visible region ranges from 400 to 700 nm. The spectrum shows peaks or bands corresponding to the wavelengths of light absorbed by the sample.