6. Consider the following proteins to answer the questions below:ProteinSize (kDa)ε at 280 nm10700014000300050000ABCD501050pl4488Red Colored?YesNoNoNoa. Sketch the UV-Vis spectrum of each pure protein (A, B, C, and D) from 240-480 nm if they were all present at 20 µM. Note: I want four clearly labeled spectraon one plot.

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Answered: Sketch the UV-Vis spectrum of each pure…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

List two situations when you would not be able to use the UV-Vis absorbance at 280 nm to determine a protein’s concentration.
Suppose you had a sample that contained equal concentrations (in unit of mg/ml) of DNA and a protein, e.g. BSA. Sketch the absorption spectrum you would expect to see in the region from 240 to 300 nm.
The α-amylase (α-AM) enzyme secreted by Aspergillus has an isoelectric point of approximately 4.2. If the purification method in problem 1 operates at pH 6.5, would the protein carry a positive charge, negative charge, or net zero charge?
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Which of the following is NOT a unit of concentration? All of these options are units of concentration. mM Moles/Liter Molar You gathered your data from Lab , and found the following values: Total Activity of lysozyme in HEW: 14 units Total Activity of lysozyme in carb 1: 21 units Total amount of protein in HEW: 70 mg Total amount of protein in Carb 1: 15 mg Calculate the extent of purification of lysozyme in Carb 1. A 7 fold B 150% C 15 fold D 70%

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Sketch the UV-Vis spectrum of each pure protein (A, B, C, and D) from 240 - 480 nm if they were all present at 20 µM. Note: I want four clearly labeled spectra on one plot.