The α-amylase (α-AM) enzyme secreted by Aspergillus has an isoelectric point of approximately 4.2. If the purification method in problem 1 operates at pH 6.5, would the protein carry a positive charge, negative charge, or net zero charge?

The pH level at which a molecule has no net electrical charge is known as the isoelectric point…

Answered: The α-amylase (α-AM) enzyme secreted by…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The α-amylase (α-AM) enzyme secreted by Aspergillus has an isoelectric point of approximately 4.2. If the purification method in problem 1 operates at pH 6.5, would the protein carry a positive charge, negative charge, or net zero charge?

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The pH level at which a molecule has no net electrical charge is known as the isoelectric point (pI).
An enzyme called α-amylase hydrolyzes α-bonds and breaks them into small-chain dextrins, which results in the breakdown of starch molecules.

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