(a) A solution containing these five proteins was adjusted to pH 7.0 and then applied to a SIZE-EXCLUSION COLUMN. Assume that these five protein molecules are spherical in shape. Which protein is likely to be eluted LAST from the column? Explain briefly. (b) In another experiment, this protein mixture was first adjusted to pH 6.7 before applying to a CATION-EXCHANGE COLUMN. (i)What is the net charge on phosphorylase b? (ii) Which of these five proteins will bind to the CATION-EXCHANGER?
The following table shows the molecular masses (Mr) and isoelectric points (pI) of five proteins:
|
Protein |
Mr |
pI |
|
Chicken egg white lysozyme |
14,000 |
11.0 |
|
Carbonic anhydrase |
30,000 |
5.4 |
|
Ovalbumin |
45,000 |
4.5 |
|
Phosphorylase b |
97,000 |
6.7 |
|
Lactate dehydrogenase |
140,000 |
7.12 |
(a) A solution containing these five proteins was adjusted to pH 7.0 and then applied to a SIZE-EXCLUSION COLUMN. Assume that these five protein molecules are spherical in shape. Which protein is likely to be eluted LAST from the column? Explain briefly.
(b) In another experiment, this protein mixture was first adjusted to pH 6.7 before applying to a CATION-EXCHANGE COLUMN.
(i)What is the net charge on phosphorylase b?
(ii) Which of these five proteins will bind to the CATION-EXCHANGER? Explain briefly.v
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