2. Peter does not tolerate lactose in milk. He bought a solution of ẞ-galactosidase where [Eo]=10.0 μM. The concentration of lactose in milk is 150 mM and pH=6.3 Enzyme activity depends on pH with constants: pKa1=6.0, pKa2=7.3 (the active form is partially protonated), KM-70 mM, Kcat=525 s¹ and enzyme loses half of its activity in 8 h. Find much enzyme solution (in mL) should Peter add to 1L of milk to reduce the concentration of lactose by 80% after 10h to maximize the enzyme usage efficiency.
2. Peter does not tolerate lactose in milk. He bought a solution of ẞ-galactosidase where [Eo]=10.0 μM. The concentration of lactose in milk is 150 mM and pH=6.3 Enzyme activity depends on pH with constants: pKa1=6.0, pKa2=7.3 (the active form is partially protonated), KM-70 mM, Kcat=525 s¹ and enzyme loses half of its activity in 8 h. Find much enzyme solution (in mL) should Peter add to 1L of milk to reduce the concentration of lactose by 80% after 10h to maximize the enzyme usage efficiency.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![2. Peter does not tolerate lactose in milk. He bought a solution of B-galactosidase where
[Eo] 10.0 µM. The concentration of lactose in milk is 150 mM and pH-6.3 Enzyme activity
depends on pH with constants: pka1-6.0, pka2=7.3 (the active form is partially protonated),
KM-70 mm, kcat = 525 s¹ and enzyme loses half of its activity in 8 h. Find much enzyme
solution (in mL) should Peter add to 1L of milk to reduce the concentration of lactose by
80% after 10h to maximize the enzyme usage efficiency.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fbc5b29d9-7e32-4c31-9f09-e42da0a8e7df%2F2b2b9626-22f4-4ebc-a5cb-4b7d02d29510%2Ff3jeaa_processed.jpeg&w=3840&q=75)
Transcribed Image Text:2. Peter does not tolerate lactose in milk. He bought a solution of B-galactosidase where
[Eo] 10.0 µM. The concentration of lactose in milk is 150 mM and pH-6.3 Enzyme activity
depends on pH with constants: pka1-6.0, pka2=7.3 (the active form is partially protonated),
KM-70 mm, kcat = 525 s¹ and enzyme loses half of its activity in 8 h. Find much enzyme
solution (in mL) should Peter add to 1L of milk to reduce the concentration of lactose by
80% after 10h to maximize the enzyme usage efficiency.
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