An octapeptide was hydrolyzed and the amino acids separated. Its amino acid composition was determined: Gly (1); Ala (2); Val (1); Lys (2); Met (1); Asp (1). After incubation with FDNB and hydrolysis, 2,4-dinitrophenylalanine was detected. NO H. N C-COOH H. ON- CH, Treatment with cyanogen bromide, which cleaves at M, resulted in two fragments: a pentapeptide containing two K residues and a tripeptide containing V, G, and A. The residues are not listed in any particular order. After treatment with trypsin, which cleaves at R and K, there were three fragments: two dipeptides and MGAV. What is the sequence of the octapeptide? Use the one-letter abbreviations for the amino acids, and do not use dashes. octapeptide sequence:
An octapeptide was hydrolyzed and the amino acids separated. Its amino acid composition was determined: Gly (1); Ala (2); Val (1); Lys (2); Met (1); Asp (1). After incubation with FDNB and hydrolysis, 2,4-dinitrophenylalanine was detected. NO H. N C-COOH H. ON- CH, Treatment with cyanogen bromide, which cleaves at M, resulted in two fragments: a pentapeptide containing two K residues and a tripeptide containing V, G, and A. The residues are not listed in any particular order. After treatment with trypsin, which cleaves at R and K, there were three fragments: two dipeptides and MGAV. What is the sequence of the octapeptide? Use the one-letter abbreviations for the amino acids, and do not use dashes. octapeptide sequence:
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.1: Introduction To Clinical Chemistry
Problem 9RQ
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![An octapeptide was hydrolyzed and the amino acids separated. Its amino acid composition was determined: Gly (1);
Ala (2); Val (1); Lys (2); Met (1); Asp (1).
After incubation with FDNB and hydrolysis, 2,4-dinitrophenylalanine was detected.
NO,
H
N -C- COOH
H
O,N
CH,
Treatment with cyanogen bromide, which cleaves at M, resulted in two fragments: a pentapeptide containing two K residues
and a tripeptide containing V, G, and A. The residues are not listed in any particular order.
After treatment with trypsin, which cleaves at R and K, there were three fragments: two dipeptides and MGAV.
What is the sequence of the octapeptide?
Use the one-letter abbreviations for the amino acids, and do not use dashes.
octapeptide sequence:](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F010b56f7-d1b9-470d-9de9-bc0dc28d0cd1%2F1dd3a374-ddb9-44ce-ac80-1da3b031ecc2%2Fbxrui9j_processed.jpeg&w=3840&q=75)
Transcribed Image Text:An octapeptide was hydrolyzed and the amino acids separated. Its amino acid composition was determined: Gly (1);
Ala (2); Val (1); Lys (2); Met (1); Asp (1).
After incubation with FDNB and hydrolysis, 2,4-dinitrophenylalanine was detected.
NO,
H
N -C- COOH
H
O,N
CH,
Treatment with cyanogen bromide, which cleaves at M, resulted in two fragments: a pentapeptide containing two K residues
and a tripeptide containing V, G, and A. The residues are not listed in any particular order.
After treatment with trypsin, which cleaves at R and K, there were three fragments: two dipeptides and MGAV.
What is the sequence of the octapeptide?
Use the one-letter abbreviations for the amino acids, and do not use dashes.
octapeptide sequence:
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