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2. You made a pH=9.8 buffer solution by mixing NaOH and glycine to give a solution that is 0.20 M in total concentration of glycine. (pkal-2.34 and pka2=9.69) a) What 2 glycine species are present in the buffer solution at this pH? (draw their structures) b) What is the concentration ratio of these 2 species? c) How many moles of each species, A and HA, is there in 500 ml of the buffer solution.

2. You made a pH=9.8 buffer solution by mixing NaOH and glycine to give a solution that is 0.20 M in total concentration of glycine. (pkal-2.34 and pka2=9.69) a) What 2 glycine species are present in the buffer solution at this pH? (draw their structures) b) What is the concentration ratio of these 2 species? c) How many moles of each species, A and HA, is there in 500 ml of the buffer solution.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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2. You made a pH=9.8 buffer solution by mixing NaOH and glycine to give a solution that is 0.20 M in total concentration of glycine. (pka1=2.34 and pka2=9.69) a) What 2 glycine species are present in the buffer solution at this pH? (draw their structures) b) What is the concentration ratio of these 2 species? c) How many moles of each species, A and HA, is there in 500 ml of the buffer solution. d) If 25 ml of 0.82 M HCl is added to 500 ml of this buffer solution. What reaction is taking place? What is the final pH of the solution? Is the solution still a buffer? e) Draw the titration curve, pH versus equivalents of OH-, for glycine and determine the pl.
Transcribed Image Text:2. You made a pH=9.8 buffer solution by mixing NaOH and glycine to give a solution that is 0.20 M in total concentration of glycine. (pka1=2.34 and pka2=9.69) a) What 2 glycine species are present in the buffer solution at this pH? (draw their structures) b) What is the concentration ratio of these 2 species? c) How many moles of each species, A and HA, is there in 500 ml of the buffer solution. d) If 25 ml of 0.82 M HCl is added to 500 ml of this buffer solution. What reaction is taking place? What is the final pH of the solution? Is the solution still a buffer? e) Draw the titration curve, pH versus equivalents of OH-, for glycine and determine the pl.
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