QUESTION 28 Consider the following properties of the listed proteins A, B and C. Affinity for glucose Yes Protein pl 3.8 Protein A Protein B Protein C 3.8 No 7.0 No Explain clearly how would you physically separte protein B from C in the best and most effective way (Separation by dialysis, isoelectric focusing and SDS PAGE is NOT an option and cannot be use as answeer choice) Eor the tnolhar press Al T+E10 (PO or Al T+EN+F10 (Mac
QUESTION 28 Consider the following properties of the listed proteins A, B and C. Affinity for glucose Yes Protein pl 3.8 Protein A Protein B Protein C 3.8 No 7.0 No Explain clearly how would you physically separte protein B from C in the best and most effective way (Separation by dialysis, isoelectric focusing and SDS PAGE is NOT an option and cannot be use as answeer choice) Eor the tnolhar press Al T+E10 (PO or Al T+EN+F10 (Mac
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question 28
 alt="QUESTION 28
Consider the following properties of the listed proteins A, B and C.
Protein
pI
Affinity
for glucose
Yes
3.8
Protein A
Protein B
Protein C
3.8
7.0
No
_No
Explain clearly how would you physically separte protein B from C in the best and most effective way.
(Separation by dialysis, isoelectric focusing and SDS PAGE is NOT an option and cannot be use as answeer choice)
For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac).
BIUS Paragraph
E v A V 2
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Arial
10pt
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QUESTION 29
Explain why when non-polar molecules are placed in water, they cause the entropy of the system to decrease?
For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac).
BIUS
Paragraph
2 I o Ō O Q S2 = = = = E E x' X;
Arial
10pt
田田田国田田因
i (1)
tick Save All Answers to save all answers.
Save All Anwers
77 d
Sh St
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Transcribed Image Text:QUESTION 28
Consider the following properties of the listed proteins A, B and C.
Protein
pI
Affinity
for glucose
Yes
3.8
Protein A
Protein B
Protein C
3.8
7.0
No
_No
Explain clearly how would you physically separte protein B from C in the best and most effective way.
(Separation by dialysis, isoelectric focusing and SDS PAGE is NOT an option and cannot be use as answeer choice)
For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac).
BIUS Paragraph
E v A V 2
工*厄启asc===三 三 x X,深|ST Te
Arial
10pt
Ω Θ
田
田田田
国田用 国す()
QUESTION 29
Explain why when non-polar molecules are placed in water, they cause the entropy of the system to decrease?
For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac).
BIUS
Paragraph
2 I o Ō O Q S2 = = = = E E x' X;
Arial
10pt
田田田国田田因
i (1)
tick Save All Answers to save all answers.
Save All Anwers
77 d
Sh St
Mycour
Expert Solution

Step 1
The isoelectric point of a protein is the pH at which the net charge of the protein is zero. At this point, the protein acts as a zwitter ion and is in the most stable form. The net charge of the protein depends on the pKa values of the side chains of the amino acids that form the protein.
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