- Draw the structure of the pentapeptide Gln-Trp-His-Glu-Tyr that would predominate in aqueous solution at pH=10.3. (Relevant pKa values are: 2.2 4.3, 6.0, 9.8, and 10.1)

Q: Two peptide segments are shown below. Predict which one would have the most negative AG when going…

A: Protein folding is a spontaneous reaction by which most of the proteins fold inside the cell without…

Q: 3. A mutation that changes an alanine residue in the interior of a protein to a valine is found to…

A: Proteins are composed of twenty standard amino acids that differ from each other in their side-chain…

Q: Identify and briefly describe three different PEGylation strategies you can use to PEGylate this…

A: The process of PEGylation improves drug solubility and decreases immunogenicity of a molecule that…

Q: (b) How ATP increases the apparent KM for substrate fructose-6-phosphate, inhibiting PFK.

A: Enzymes are the proteins which work as biological catalysts help to speed up the biochemical…

Q: At pH 7, the most predominant interaction between glucuronic acid (structure seen below) and the…

A: Electrostatic interactions are the  interactions that might be attractive or repulsive that form…

Q: The tripeptide +H3N-Leu-His-Glu-COO- has pKa  values of 2.2, 3.6, 6.0 and 9.1.  The  isoelectric…

A: Isoelectric point (pI) is the pH at which there is no net charge on the molecule. For a amino acid,…

Q: 2. Draw a dipeptide with a trans linkage and a net charge of -1 at pH 7 a. Indicate which six atoms…

A: The peptides are composed of twenty standard amino acids that differ from each other in their…

Q: Given a tripeptide Cys-His-Lys, Cys: Pk1 = 1.71; Pk2 = 10.78; PkR = 8.33 His: Pk1 = 1.82; Pk2 =…

A: pK refers to pH at which a particular group is exactly 50% ionized. A tripeptide is a peptide…

Q: peptide Lys-Glu-Trp  answer the following questions:         Draw the structure of this peptide…

A: There are 4 types of biological macromolecules: proteins, nucleic acids, lipids and…

Q: Ala-Arg-Val-His-Asp-Gln Given the polypeptide chain above Estimate the net charge of the…

A: The amino acid molecule possesses the amine (-NH2) and a carboxylic acid (-COOH) functional group…

Q: Which ligand-binding curve in the figure below would be consistent with the protein exhibiting a Ka…

A: Consider the interaction between a protein (P) and ligand (L) as shown below.Here, Ka is the…

Q: A researcher performed an SDS-PAGE experiment (with 2- mercaptoethanol) on a mixture containing 3…

A: G

Q: Consider the following peptide: alanyl-glutamyl-lysyl-cysteyl-asparagine. 1- Write the…

A: The given peptide is comprised of 5 amino acids. In order to write the name of a peptide , we add…

Q: 16. A quantitative study of the interaction of a protein with its ligand yielded the following…

A: I cannot plot the graph as that would be doing your homework.Determine KD:From the graph, read the…

Q: The following peptide that has been acetylated at the C-terminus: CH3C(O)-GEEGH-NH2 Plot the…

A: Titration is a very useful procedure to estimate the concentration of any unknown compound in a…

Q: - The following data describe the catalysis of cleavage of peptide bonds in small peptides by the…

A: Introduction: The bond that is present between the two amino acids is known as an amide bond or…

Q: A synthetic polypeptid made up of L-glutamic acid residues is in a random coil configuration at pH…

A: Hydrophobic effect: non-polar side groups that do not ionize interact with other non-polar side…

Q: TABLE 3-1 Properties and Conventions Associated with the Amino Acids pk, values Amino acid…

A: pI refers to the isoelectric point or the pH at which the amino acid carries a positive charge and a…

Q: Peptide A: AFEQHSR Calculate the isoelectric point of Peptide A using the pKa values in Table 4-1 of…

A: A peptide is a short chain of amino acid residue linked via a peptide bond. Recall that: Amino acid…

Q: Calculate the net charge on the following tripeptides at pH 5.0: (a) Leu-His-Asp [0] (b)…

A: Recall that: Amino acid sequences are written with N-terminal amino acid on the left and C-terminal…

Q: whereas protein B does not absorb at 340 nm. What absorbance will be observed when light at 340 nm…

A: Given, ε (extinction coefficient) = 6440 M-1 cm-1 l (path length) = 5 mm = 0.5 cm c (concentration)…

Q: dentify and describe how you would PEGylate this peptide at its N- terminal amine. Discuss the…

A: The process of PEGylation improves drug solubility and decreases immunogenicity of a molecule that…

Q: At pH 7, draw the structure of Arg-Tyr-Gln-Glu-Lys. 2. What’s the charge of this peptide at pH 12?…

A: 1. At pH 7, the Arg-Tyr-Gln-Glu-Lys peptide would have the following structure: H       H    H…

Q: Identify and briefly describe the different PEGylation strategies you can use to PEGylate this…

A: PEGylation refers to the process where polyethylene glycol polymer is chemically added to a protein…

Q: Determine the net charge on the following pentapeptide "Lys-Ala-His-Asp-Ser" at: a. pH 1.0 b. pH…

A: The estimated charge on the given Lys-Ala-His-Asp-Ser peptide is +3.At low pH, the amino acid is…

Q: Describe the concept of isoelectric point and calculate the PI for isoleucine?

A: Isoelectric point is the pH value at which the charge on an amino acid is zero.

Q: 1. The graph given represents a size-exclusion chromatogram after the refolding of the hemoglobin…

A: Chromatography is a technique of protein purification in which protein can be separated based on its…