64 kDa, Tetramer (a B)2 32 kDa, Dimer αβ 16 kDa, Monomer or B a Elution Volume (mL) Absorban ce at 280 nm
64 kDa, Tetramer (a B)2 32 kDa, Dimer αβ 16 kDa, Monomer or B a Elution Volume (mL) Absorban ce at 280 nm
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Hemoglobin is considered to be a tetrameric complex with a 64 kDa (α β)2. When attempting to purify hemoglobin, we must first purify the α and β monomers (about 16 kDa each) to prepare the tetramer. This is formed from the dimer intermediate: 2 α + 2 β -> 2 αβ -> (α β)2. The graph given represents a size-exclusion chromatogram after the refolding of the hemoglobin tetramer
Using the size-exclusion chromatogram given,
1. Draw an SDS-Page Gel with a reducing agent such as BME using the three peaks listed on the graph.
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