64 kDa, Tetramer (a B)2 32 kDa, Dimer αβ 16 kDa, Monomer or B a Elution Volume (mL) Absorban ce at 280 nm
64 kDa, Tetramer (a B)2 32 kDa, Dimer αβ 16 kDa, Monomer or B a Elution Volume (mL) Absorban ce at 280 nm
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Hemoglobin is considered to be a tetrameric complex with a 64 kDa (α β)2. When attempting to purify hemoglobin, we must first purify the α and β monomers (about 16 kDa each) to prepare the tetramer. This is formed from the dimer intermediate: 2 α + 2 β -> 2 αβ -> (α β)2. The graph given represents a size-exclusion chromatogram after the refolding of the hemoglobin tetramer
Using the size-exclusion chromatogram given,
1. Draw an SDS-Page Gel with a reducing agent such as BME using the three peaks listed on the graph.
![64 kDa, Tetramer
(α β)2
32 kDa, Dimer
αβ
16 kDa, Monomer
a or ß
a
Elution Volume (mL)
Absorban ce at 280 nm](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9b60de5f-8fb0-4e3b-8258-2de6c1a93a8c%2Fd5e80104-2cd8-40da-b683-ac1a8b293edf%2Fjfc0yzo_processed.png&w=3840&q=75)
Transcribed Image Text:64 kDa, Tetramer
(α β)2
32 kDa, Dimer
αβ
16 kDa, Monomer
a or ß
a
Elution Volume (mL)
Absorban ce at 280 nm
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