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6. You weigh 5 milligrams of protein and dissolve it in 1.0 mL of water to make a stock solution with a concentration of 10 mg/mL. You plan to load 10ug of protein in each lane on your SDS PAGE. To do this, you would like to have 10µg in 5 ul so that you can load 5 µl into each well. (5 μl is easy to pipette and fits into a well.) 6a. Using your 5 mg/mL stock solution, you wish to make 100μL of the correct concentration of protein to load. How do you make this working solution? (Yes- you would want this to be in 1x sample buffer, but that makes this question more complicated. So for this question just make the protein in water.) Volume of 10mg/ml Final Volume 100μL of protein 6b. This is a 1: dilution.

6. You weigh 5 milligrams of protein and dissolve it in 1.0 mL of water to make a stock solution with a concentration of 10 mg/mL. You plan to load 10ug of protein in each lane on your SDS PAGE. To do this, you would like to have 10µg in 5 ul so that you can load 5 µl into each well. (5 μl is easy to pipette and fits into a well.) 6a. Using your 5 mg/mL stock solution, you wish to make 100μL of the correct concentration of protein to load. How do you make this working solution? (Yes- you would want this to be in 1x sample buffer, but that makes this question more complicated. So for this question just make the protein in water.) Volume of 10mg/ml Final Volume 100μL of protein 6b. This is a 1: dilution.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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6. You weigh 5 milligrams of protein and dissolve it in 1.0 mL of water to make a stock solution with a concentration of 10 mg/mL. You plan to load 10ug of protein in each lane on your SDS PAGE. To do this, you would like to have 10µg in 5 ul so that you can load 5 µl into each well. (5 μl is easy to pipette and fits into a well.) 6a. Using your 5 mg/mL stock solution, you wish to make 100μL of the correct concentration of protein to load. How do you make this working solution? (Yes- you would want this to be in 1x sample buffer, but that makes this question more complicated. So for this question just make the protein in water.) Volume of 10mg/ml Final Volume 100μL of protein 6b. This is a 1: Q 2 Body* W #3 dilution. E $ 4 A R B % 5 MacBook Pro I U T A 6 Y & U 8 (B
Transcribed Image Text:6. You weigh 5 milligrams of protein and dissolve it in 1.0 mL of water to make a stock solution with a concentration of 10 mg/mL. You plan to load 10ug of protein in each lane on your SDS PAGE. To do this, you would like to have 10µg in 5 ul so that you can load 5 µl into each well. (5 μl is easy to pipette and fits into a well.) 6a. Using your 5 mg/mL stock solution, you wish to make 100μL of the correct concentration of protein to load. How do you make this working solution? (Yes- you would want this to be in 1x sample buffer, but that makes this question more complicated. So for this question just make the protein in water.) Volume of 10mg/ml Final Volume 100μL of protein 6b. This is a 1: Q 2 Body* W #3 dilution. E $ 4 A R B % 5 MacBook Pro I U T A 6 Y & U 8 (B
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