In this question, you need to discuss the results you obtained from the BSA standards to generate your standard curve. Are you confident in the absorbance values you measured from the BSA standards? Was there variability between your duplicates? Comment on the shape of your standard curve and note the highest absorbance you would consider for interpolating the concentration of an unknown sample.
In this question, you need to discuss the results you obtained from the BSA standards to generate your standard curve. Are you confident in the absorbance values you measured from the BSA standards? Was there variability between your duplicates? Comment on the shape of your standard curve and note the highest absorbance you would consider for interpolating the concentration of an unknown sample.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
In this question, you need to discuss the results you obtained from the BSA standards to generate your standard curve.
Are you confident in the absorbance values you measured from the BSA standards? Was there variability between your duplicates? Comment on the shape of your standard curve and note the highest absorbance you would consider for interpolating the concentration of an unknown sample.
Transcribed Image Text:Absorbance (A540)
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0
5
Absorbance (A540) vs Concentration of protein (mg/ml)
10
15
Concentration of protein (mg/ml)
20
25
-Replicate 1
-Replicate 2
Average between replicate 1/2
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