questions. Generic Standard Curve 0.7 y = 0.9425x- 0.0034 0.6 0.5 04 0.3 02 0.1 0.1 02 0.3 0.4 0.5 0.6 0.7 Concentration (mg/ml) - Please describe two ways in which you could use this standard curve to determine the concentration of a pigment in an unknown sample. %231: #2: -What is your rough estimation of the concentration if you determine the absorbance of a sample is 0.150? Absorbance

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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**Text Transcription for Educational Website**

The standard curve provided below will allow you to answer the remaining questions.

**Graph Explanation**

The graph is titled "Generic Standard Curve." It shows a linear relationship between concentration (x-axis) in mg/mL and absorbance (y-axis). The equation of the line is \( y = 0.9425x - 0.0034 \). The x-axis is labeled from 0 to 0.7, and the y-axis ranges from 0 to 0.7 as well. Data points representing various concentrations and their corresponding absorbance values are plotted, and a best-fit line is drawn through these points.

**Instructions for Use**

- Please describe two ways in which you could use this standard curve to determine the concentration of a pigment in an unknown sample.

  **#1:**

  **#2:**

- What is your rough estimation of the concentration if you determine the absorbance of a sample is 0.150?
Transcribed Image Text:**Text Transcription for Educational Website** The standard curve provided below will allow you to answer the remaining questions. **Graph Explanation** The graph is titled "Generic Standard Curve." It shows a linear relationship between concentration (x-axis) in mg/mL and absorbance (y-axis). The equation of the line is \( y = 0.9425x - 0.0034 \). The x-axis is labeled from 0 to 0.7, and the y-axis ranges from 0 to 0.7 as well. Data points representing various concentrations and their corresponding absorbance values are plotted, and a best-fit line is drawn through these points. **Instructions for Use** - Please describe two ways in which you could use this standard curve to determine the concentration of a pigment in an unknown sample. **#1:** **#2:** - What is your rough estimation of the concentration if you determine the absorbance of a sample is 0.150?
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