COMPLETE ALL CALCULATIONS REQUIRED FOR TABLE 1 ( For all measurements, the [PNPP] concentration will be 600 μM in a 1 ml final volume.) PNPP (substrate) stock solution: 100 mM PNPP in buffer 2. IN YOUR LAB NOTEBOOK, DRAW YOUR PREDICTION OF WHAT THE FOLLOWING GRAPHS MIGHT LOOK LIKE a. pH vs. V0 b. TEMP vs. V0
COMPLETE ALL CALCULATIONS REQUIRED FOR TABLE 1 ( For all measurements, the [PNPP] concentration will be 600 μM in a 1 ml final volume.) PNPP (substrate) stock solution: 100 mM PNPP in buffer 2. IN YOUR LAB NOTEBOOK, DRAW YOUR PREDICTION OF WHAT THE FOLLOWING GRAPHS MIGHT LOOK LIKE a. pH vs. V0 b. TEMP vs. V0
Chapter20: Iv Medication And Titration Calculations
Section: Chapter Questions
Problem 1.6P
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Please answer these question. I need these for lab.
PRE-LAB ASSIGNMENT – (YOU WILL NOT BE ALLOWED TO BEGIN WORK ON LAB
WITHOUT THE COMPLETED CALCULATIONS)
1. COMPLETE ALL CALCULATIONS REQUIRED FOR TABLE 1 ( For all measurements, the [PNPP] concentration will be 600 μM in a 1 ml final volume.) PNPP (substrate) stock solution: 100 mM PNPP in buffer
2. IN YOUR LAB NOTEBOOK, DRAW YOUR PREDICTION OF WHAT THE
FOLLOWING GRAPHS MIGHT LOOK LIKE
a. pH vs. V0
b. TEMP vs. V0
3. OUTLINE THE BEST/MOST EFFICIENT WAY TO PREPARE
![Table 1
Total volume (ul) PNPP stock (ul)
1000
Buffer (μl)
Enzyme (ul)
5
Final [PNPP] (uM)
600](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe31029a8-64b8-4001-b02b-a4ced2e71261%2F7293c6ca-d96d-4c84-8829-19cabd420bb4%2F9xkejn_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Table 1
Total volume (ul) PNPP stock (ul)
1000
Buffer (μl)
Enzyme (ul)
5
Final [PNPP] (uM)
600
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