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COMPLETE ALL CALCULATIONS REQUIRED FOR TABLE 1 ( For all measurements, the [PNPP] concentration will be 600 μM in a 1 ml final volume.) PNPP (substrate) stock solution: 100 mM PNPP in buffer 2. IN YOUR LAB NOTEBOOK, DRAW YOUR PREDICTION OF WHAT THE FOLLOWING GRAPHS MIGHT LOOK LIKE a. pH vs. V0 b. TEMP vs. V0

COMPLETE ALL CALCULATIONS REQUIRED FOR TABLE 1 ( For all measurements, the [PNPP] concentration will be 600 μM in a 1 ml final volume.) PNPP (substrate) stock solution: 100 mM PNPP in buffer 2. IN YOUR LAB NOTEBOOK, DRAW YOUR PREDICTION OF WHAT THE FOLLOWING GRAPHS MIGHT LOOK LIKE a. pH vs. V0 b. TEMP vs. V0

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Please answer these question. I need these for lab.

PRE-LAB ASSIGNMENT – (YOU WILL NOT BE ALLOWED TO BEGIN WORK ON LAB
WITHOUT THE COMPLETED CALCULATIONS)


1. COMPLETE ALL CALCULATIONS REQUIRED FOR TABLE 1 ( For all measurements, the [PNPP] concentration will be 600 μM in a 1 ml final volume.) PNPP (substrate) stock solution: 100 mM PNPP in buffer

2. IN YOUR LAB NOTEBOOK, DRAW YOUR PREDICTION OF WHAT THE
FOLLOWING GRAPHS MIGHT LOOK LIKE
a. pH vs. V0
b. TEMP vs. V0
3. OUTLINE THE BEST/MOST EFFICIENT WAY TO PREPARE

**Table 1** | Total volume (μl) | PNPP stock (μl) | Buffer (μl) | Enzyme (μl) | Final [PNPP] (μM) | |-------------------|-----------------|-------------|-------------|-------------------| | 1000 | | | 5 | 600 | **Explanation:** This table presents information related to an experimental setup. - **Total volume (μl):** The total volume of the solution used in the experiment, which is 1000 microliters. - **PNPP stock (μl) and Buffer (μl):** These columns are left blank and typically indicate that specific volumes need to be calculated or are variable based on the experimental needs. - **Enzyme (μl):** Indicates that 5 microliters of the enzyme are to be used in the experiment. - **Final [PNPP] (μM):** The final concentration of PNPP in the solution is 600 micromolar. This table structure is commonly used in biochemical experiments involving enzyme reactions to provide a quick reference for the volumes and concentrations needed.
Transcribed Image Text:**Table 1** | Total volume (μl) | PNPP stock (μl) | Buffer (μl) | Enzyme (μl) | Final [PNPP] (μM) | |-------------------|-----------------|-------------|-------------|-------------------| | 1000 | | | 5 | 600 | **Explanation:** This table presents information related to an experimental setup. - **Total volume (μl):** The total volume of the solution used in the experiment, which is 1000 microliters. - **PNPP stock (μl) and Buffer (μl):** These columns are left blank and typically indicate that specific volumes need to be calculated or are variable based on the experimental needs. - **Enzyme (μl):** Indicates that 5 microliters of the enzyme are to be used in the experiment. - **Final [PNPP] (μM):** The final concentration of PNPP in the solution is 600 micromolar. This table structure is commonly used in biochemical experiments involving enzyme reactions to provide a quick reference for the volumes and concentrations needed.
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