Determination of Creatinine in Serum and Urine Experiment; Question: Why are using this materials/chemicals in this experiment? *10% sodium tungstate *0.75 M NaOH Creatinine depot standard *0.67 N H2SO4 *Picric acid (0.04 M) Procedure: 3 mL of water, 1 mL of 10% sodium tungstate, 2 mL of 0.67 N H2SO4 are added onto the serum (2 mL), mixed well, and filtered after 5-10 minutes (protein-free serum filtrate). 3 mL of this filtrate is used for the test. All tubes are mixed, incubated at room temperature for 20 minutes and read against the reagent blank at 520 nm. First, the serum volume in the test tube is calculated. Since working with 3 mL standard, the absorbance of the standard tube belongs to 30 µg creatinine.
Determination of Creatinine in Serum and Urine Experiment; Question: Why are using this materials/chemicals in this experiment? *10% sodium tungstate *0.75 M NaOH Creatinine depot standard *0.67 N H2SO4 *Picric acid (0.04 M) Procedure: 3 mL of water, 1 mL of 10% sodium tungstate, 2 mL of 0.67 N H2SO4 are added onto the serum (2 mL), mixed well, and filtered after 5-10 minutes (protein-free serum filtrate). 3 mL of this filtrate is used for the test. All tubes are mixed, incubated at room temperature for 20 minutes and read against the reagent blank at 520 nm. First, the serum volume in the test tube is calculated. Since working with 3 mL standard, the absorbance of the standard tube belongs to 30 µg creatinine.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Determination of Creatinine in Serum and Urine Experiment;
Question: Why are using this materials/chemicals in this experiment?
*10% sodium tungstate
*0.75 M NaOH Creatinine depot standard
*0.67 N H2SO4
*Picric acid (0.04 M)
Procedure:
3 mL of water, 1 mL of 10% sodium tungstate, 2 mL of 0.67 N H2SO4 are added onto the serum (2 mL), mixed well, and filtered after 5-10 minutes (protein-free serum filtrate). 3 mL of this filtrate is used for the test.
All tubes are mixed, incubated at room temperature for 20 minutes and read against the reagent blank at 520 nm.
First, the serum volume in the test tube is calculated.
Since working with 3 mL standard, the absorbance of the standard tube belongs to 30 µg creatinine.
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