Lab 3: Concentrations/Standard Curve Lab Data and Questions Activity 3: MAKING DILUTIONS AND STANDARD CURVE Final concentration of BSA (bovine serum albumin) (ug/ml) 2000ug/ml Tube Abs595nm measured by spectrophotometer 1 0.541 1000ug/ml 0.423 3. 500ug/ml 0.247 4 250ug/ml O.160 Graph 1: Plot a standard curve of Absorbance (A) vs. concentration of BSA (ug/ml) in the graph below. Draw a best fit line. You will use this graph for Activity 4. Be sure to label your axis. (200 (250 e. (a50, 0A60 01 a50 1. What is the equation of the best fit line for the graph above? 500 2000 1000 Concentrition of BS Absorbance (A)
Lab 3: Concentrations/Standard Curve Lab Data and Questions Activity 3: MAKING DILUTIONS AND STANDARD CURVE Final concentration of BSA (bovine serum albumin) (ug/ml) 2000ug/ml Tube Abs595nm measured by spectrophotometer 1 0.541 1000ug/ml 0.423 3. 500ug/ml 0.247 4 250ug/ml O.160 Graph 1: Plot a standard curve of Absorbance (A) vs. concentration of BSA (ug/ml) in the graph below. Draw a best fit line. You will use this graph for Activity 4. Be sure to label your axis. (200 (250 e. (a50, 0A60 01 a50 1. What is the equation of the best fit line for the graph above? 500 2000 1000 Concentrition of BS Absorbance (A)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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What is the equation of the best fit line for the graph above
Transcribed Image Text:Lab 3: Concentrations/Standard Curve Lab
Data and Questions
Activity 3: MAKING DILUTIONS AND STANDARD CURVE
Final concentration of BSA
(bovine serum albumin)
(ug/ml)
2000ug/ml
Tube
Abs595nm measured by
spectrophotometer
1
0.541
1000ug/ml
0.423
3.
500ug/ml
0.247
4
250ug/ml
O.160
Graph 1: Plot a standard curve of Absorbance (A) vs. concentration of BSA (ug/ml) in the graph
below. Draw a best fit line. You will use this graph for Activity 4. Be sure to label your axis.
(200
(250
e.
(a50, 0A60
01
a50
1. What is the equation of the best fit line for the graph above?
500
2000
1000
Concentrition of BS
Absorbance (A)
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