Which of the following components of Km is largest in magnitude? k2 O k-1 O k1 O k-2
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- Figure 3: ● ● ● ● ● ● KDa ● 97.4 66.2 45.0 ● 31.0- 21.5 14.4 S-1 p-1 S-2 2-0 This figure was generated by centrifuging a pura sample of protein, removing the supernatant, and resuspending the pellet in the same volume as the supernatant to allow direct comparison. The supernatant and pellet samples were then prepared for SDS-PAGE identically and run via normal SDS-PAGE procedures. In the figure, "s" means supernatant and "p" means pellet. The text or number after the dash represents a different condition. For example, s-1 and p-1 are the supernatant and pellet samples under condition 1. It is not shown, but under wild-type conditions, essentially all of the protein is found in the supernatant. S-3 ● What does the intensity of each band represent? ● Would you find soluble protein in the supernatant or pellet? Why? Would you find aggregated protein in the supernatant or pellet? Why? For each condition (there are 5 different conditions), is there a higher percentage of the total protein…but why is the answers all x 1000 at the end? for example day 1 is 2.025×1000=2025CFU/ml. should the answer not stay at 2.025?Match the following formulas with their correct descriptions form the drop down menu provided. Formulas 1. D subscript p equals N over A 2. D subscript p equals N over V 3. increment N equals open parentheses N plus I close parentheses minus open parentheses M plus E close parentheses 4. g r equals fraction numerator increment N over denominator increment t end fraction 5. c g r equals fraction numerator increment N over denominator N end fraction Descriptions: Used to calculate the rate of change in the population in relation to its initial population number Answer Used to calculate the change in population size Answer Used to calculate the number of individuals occupying a volume of air or water Answer Used to calculate the change in the number of individuals in a given population over a period of time. Answer Use to calculate the number of individuals occupying a land area Answer
- Succinic acid is produced by Actinobacillus succinogenes with the following medium composition (g/L): glucose, 50; yeast extract, 5; NaCl, l; Na2HPO4, 0.3; NaH2PO4, 1.4; K2HPO4, 1.5; MgC12.6H20, 0.2; CaC12.2H20, 0.23. The pH is controlled and maintained at 3.63. Estimate the range of upper limit, if 0.8% of deadband is applied. With a schematic diagram, explain how to maintain the pH throughout the fermentation process.What does an A280 of 0.000 indicate? What does this reading indicate in the context of your column chromatography? Are there still proteins adhering to the column? Do you know anything about the proteins which are still adhering to the column?Your pharmacy has on hand Tetracycline 250 mg/5 mL liquid.You receive a prescription for Tetracycline syrup 500 mg qid for 7 days. How many total milliliters would you dispense? Your pharmacy has on hand Prednisone 5 mg tablets.You receive a prescription for Prednisone 5 mg ii tabs stat and qid x 1 day, then ii tabs tid x 2 days, then ii tabs bid x 2 days, then i tab tid x 3 days, then i tab bid x 3days, then i tab qd x 4 days How many tablets would you dispense for this prescription? How many days will the patient take this medication for? Your pharmacy has on hand Dilantin 100mg capsules.You receive a prescription for Dilantin 100 mg 1 cap po in the AM & PM, 200mg (2 caps) po at noon, and 300mg (3 caps) po at hs. Dispense a 30 day supply. (using the 100mg capsules you have in stock) How many capsules will you dispense? Your pharmacy has on hand Dakins Irrigation Solution 1 liter.You receive a prescription for Dakins Irrigation Solution Irrigate chest tube with 100 mL…
- I want clear handwritten solution of both parts....i will up voteWhat are the x and y values that you have input in the excel? I thought the x would be from the concentation and the y is from the A595 of sample minus A595 of blank but the linear equation that shows up in mine is different. It's y = 0.0443x + 0.0148.The replicate results (mg/L) obtained from calcium determination of a blood sample using atomic absorption spectrophotometry (AAS) and a new colorimetric method were as following: AAS : 11.2, 11.2, 10.6, 11.2, 9.7 and 10.0 Colorimetric : 9.2, 10.5, 9.7, 11.5, 11.6, 9.3, 10.1 and 11.2 Determine whether the mean of both methods have a significant difference at 95% confidence level.
- When deoxyhemoglobin was first isolated in crystalline form, the researcher who did so noted that the crystals changed color from purple to red and also changed shape as he observed them under a microscope. What is happening on the molecular level? Hint: The crystals were mounted on a microscope slide with a loosely fitting cover slip.Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206What is the type of the following variable? double num = 12.345; char int string O double « Previous