1/[S] The figure is a Lineweaver-Burk plot. Which of the following is equal to the x- intercept in a LB plot? Choose the one best answer. -Km/Vmax 1/Vmax 1/Km O-1/Vmax 0-1/Km
1/[S] The figure is a Lineweaver-Burk plot. Which of the following is equal to the x- intercept in a LB plot? Choose the one best answer. -Km/Vmax 1/Vmax 1/Km O-1/Vmax 0-1/Km
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
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**Understanding Lineweaver-Burk Plots**
**The figure is a Lineweaver-Burk plot. Which of the following is equal to the x-intercept in a LB plot? Choose the one best answer.**
- ( ) -Km/Vmax
- ( ) 1/Vmax
- ( ) 1/Km
- ( ) -1/Vmax
- ( ) -1/Km **(Correct Answer)**
**Explanation of the Diagram:**
The Lineweaver-Burk plot, also known as the double reciprocal plot, is a graphical representation of the Lineweaver-Burk equation of enzyme kinetics. In this type of plot, the reciprocal of the reaction rate (1/V) is plotted against the reciprocal of the substrate concentration (1/[S]).
**Key features of the plot include:**
- **Y-axis (vertical):** 1/V (reciprocal of reaction velocity)
- **X-axis (horizontal):** 1/[S] (reciprocal of substrate concentration)
- The y-intercept of the plot equals 1/Vmax.
- The x-intercept is equal to -1/Km.
In the given problem, the correct answer to the question about what the x-intercept represents in a Lineweaver-Burk plot is "-1/Km".
**Educational Importance:**
Understanding the Lineweaver-Burk plot is essential for analyzing enzyme kinetics. By interpreting the intercepts, one can determine important kinetic parameters, such as Km (Michaelis constant) and Vmax (maximum reaction velocity), which provide insights into enzyme affinity and catalytic capabilities.
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This transcription provides a clear explanation suited for an educational website, detailing the elements of the plot and emphasizing the importance of understanding these concepts in enzyme kinetics.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F07b00a25-4bbc-4603-8563-821c16d74641%2F0dbd07f2-bc9c-42b6-ad24-0ef93aae3a9f%2Fg5k5h2hj_processed.jpeg&w=3840&q=75)
Transcribed Image Text:---
**Understanding Lineweaver-Burk Plots**
**The figure is a Lineweaver-Burk plot. Which of the following is equal to the x-intercept in a LB plot? Choose the one best answer.**
- ( ) -Km/Vmax
- ( ) 1/Vmax
- ( ) 1/Km
- ( ) -1/Vmax
- ( ) -1/Km **(Correct Answer)**
**Explanation of the Diagram:**
The Lineweaver-Burk plot, also known as the double reciprocal plot, is a graphical representation of the Lineweaver-Burk equation of enzyme kinetics. In this type of plot, the reciprocal of the reaction rate (1/V) is plotted against the reciprocal of the substrate concentration (1/[S]).
**Key features of the plot include:**
- **Y-axis (vertical):** 1/V (reciprocal of reaction velocity)
- **X-axis (horizontal):** 1/[S] (reciprocal of substrate concentration)
- The y-intercept of the plot equals 1/Vmax.
- The x-intercept is equal to -1/Km.
In the given problem, the correct answer to the question about what the x-intercept represents in a Lineweaver-Burk plot is "-1/Km".
**Educational Importance:**
Understanding the Lineweaver-Burk plot is essential for analyzing enzyme kinetics. By interpreting the intercepts, one can determine important kinetic parameters, such as Km (Michaelis constant) and Vmax (maximum reaction velocity), which provide insights into enzyme affinity and catalytic capabilities.
---
This transcription provides a clear explanation suited for an educational website, detailing the elements of the plot and emphasizing the importance of understanding these concepts in enzyme kinetics.
![**Understanding the Lineweaver-Burk Plot in Enzyme Kinetics**
The Lineweaver-Burk plot is a graphical representation of enzyme kinetics, allowing for a clear visualization of key parameters in enzyme-catalyzed reactions.
### Diagram Explanation
The plot depicted is a double reciprocal transformation of the Michaelis-Menten equation and graphs 1/v (reciprocal of velocity) against 1/[S] (reciprocal of substrate concentration). Key elements in the diagram include:
- **X-axis:** 1/[S] (reciprocal of substrate concentration)
- **Y-axis:** 1/v (reciprocal of reaction velocity)
- **Y-intercept:** Represents 1/Vmax, where Vmax is the maximum reaction velocity.
- **X-intercept:** Represents -1/Km, where Km is the Michaelis constant.
- **Slope:** The slope of the line is Km/Vmax.
### Sample Question
**Question:**
The figure is a Lineweaver-Burk plot. Which of the following is equal to the x-intercept in a Lineweaver-Burk plot? Choose the one best answer.
- ○ -Km/Vmax
- ○ 1/Vmax
- ○ 1/Km
**Answer:**
- The correct answer is: **-1/Km**
Understanding these parameters is crucial for interpreting enzyme kinetics and for applications in biochemistry and pharmacology.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F07b00a25-4bbc-4603-8563-821c16d74641%2F0dbd07f2-bc9c-42b6-ad24-0ef93aae3a9f%2F5q2nze_processed.jpeg&w=3840&q=75)
Transcribed Image Text:**Understanding the Lineweaver-Burk Plot in Enzyme Kinetics**
The Lineweaver-Burk plot is a graphical representation of enzyme kinetics, allowing for a clear visualization of key parameters in enzyme-catalyzed reactions.
### Diagram Explanation
The plot depicted is a double reciprocal transformation of the Michaelis-Menten equation and graphs 1/v (reciprocal of velocity) against 1/[S] (reciprocal of substrate concentration). Key elements in the diagram include:
- **X-axis:** 1/[S] (reciprocal of substrate concentration)
- **Y-axis:** 1/v (reciprocal of reaction velocity)
- **Y-intercept:** Represents 1/Vmax, where Vmax is the maximum reaction velocity.
- **X-intercept:** Represents -1/Km, where Km is the Michaelis constant.
- **Slope:** The slope of the line is Km/Vmax.
### Sample Question
**Question:**
The figure is a Lineweaver-Burk plot. Which of the following is equal to the x-intercept in a Lineweaver-Burk plot? Choose the one best answer.
- ○ -Km/Vmax
- ○ 1/Vmax
- ○ 1/Km
**Answer:**
- The correct answer is: **-1/Km**
Understanding these parameters is crucial for interpreting enzyme kinetics and for applications in biochemistry and pharmacology.
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